- Clone
- W18079D (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Eukaryotic Translation Initiation Factor 4 Gamma 1, EIF-4-Gamma 1, EIF-4-Gamma 1, EIF-4G1, EIF4GI, EIF4F, EIF4G, p220, PARK18
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![W18079D_PURE_eIF4G1_Antibody_1_091619.png W18079D_PURE_eIF4G1_Antibody_1_091619.png](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W18079D_PURE_eIF4G1_Antibody_1_091619.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-eIF4G1 Antibody, clone W18079D, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker. -
HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with methanol for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with a 1:500 dilution (1.0 µg/mL) of either Purified Rat IgG2a, κ Isotype Ctrl Antibody (Cat. No. 400502, panel A) or Purified anti-eIF4G1 Antibody, clone W18079D (Panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 Goat anti-rat IgG Antibody (Cat. No. 405422) at 2.0 µg/mL. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective. -
Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.0 µg of Purified Rat IgG2a, κ Isotype Ctrl Antibody (Cat. No. 400502) or Purified anti-eIF4G1 Antibody, clone W18079D. The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with a rabbit control antibody against a separate epitope of eIF4G1. Lane M: Molecular Weight marker.
Cat # | Size | Price | Save |
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933301 | 25 µg | ¥22,220 | |
933302 | 100 µg | ¥55,660 |
Eukaryotic initiation factor 4 gamma 1 (eIF4G1), the most abundant isoform of the eIF4G family, is an important component of the eIF4F complex, which plays a role in cap-dependent protein synthesis. eIF4G1 is a scaffolding protein that provides a platform for other translation initiation factors, including eIF4E, eIF4A, and the eIF3 complex. Protein synthesis is promoted in the presence of growth factors and hormones through the stimulation of tyrosine kinase receptors, which in turn activate the mTOR pathway. mTOR phosphorylates 4EBP1, which leads to the binding of eIF4E to eIF4G1 and thereby facilitates the assembly of the eIF4F complex. In addition to the initiation factors, eIF4G1 interacts with poly-A-binding protein (PABP), which promotes the circularization of mRNA by bridging the 5’ cap to the 3’ poly-A tail.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Partial recombinant human eIF4G1 protein (amino acids 101-350)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, IP - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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This clone recognizes both human and mouse eIF4G1.
Both Triton X-100 and methanol permeabilization produced strong and specific signal in ICC testing. - RRID
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AB_2814641 (BioLegend Cat. No. 933301)
AB_2814642 (BioLegend Cat. No. 933302)
Antigen Details
- Structure
- eIF4G1 is a 1599 amino acid protein with a predicted molecular weight of 175 kD.
- Distribution
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Cytosol/Ubiquitously expressed
- Function
- Translation
- Biology Area
- Protein Synthesis
- Molecular Family
- Scaffold Proteins
- Antigen References
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- Hentze M. 1997. Science. 275:500.
- Tarun S, et al. 1997. Proc Natl Acad Sci USA. 94:9046.
- Gross JD, et al. 2003. Cell. 115:739-50.
- Hinton T, et al. 2007. J. Biol. Chem. 282:1695.
- Gene ID
- 1981 View all products for this Gene ID
- UniProt
- View information about eIF4G1 on UniProt.org
Related FAQs
Other Formats
View All eIF4G1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-eIF4G1 | W18079D | WB,ICC,IP |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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