- Clone
- QA19A84 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Gamma-aminobutyric acid type B receptor subunit 2, gb2, GABA-BR2, GABA-B-R2, GABA-B receptor 2, GABA-B receptor, R2 subunit, G protein-coupled receptor 51, G-protein coupled receptor 51, gamma-aminobutyric acid B receptor 2, GABABR2, GABBR2
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![QA19A84_PURE_GABABR2_Antibody_013122 QA19A84_PURE_GABABR2_Antibody_013122](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/QA19A84_PURE_GABABR2_Antibody_013122.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Total tissue lysates (15 µg total protein) from rat brain (Lane 1), mouse brain (Lane 2), human brain (Lane 3), and human kidney (Lane 4, negative control) were resolved by 4-12 % Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL of purified anti-GABABR2 recombinant (clone QA19A84) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at 1:3000 dilution. Direct-Blot™ HRP anti-β actin (Cat. No. 664803) was used as a loading control at a 1:50000 dilution (lower). Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection reagent. Lane M: Molecular weight ladder
Cat # | Size | Price | Save |
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600251 | 25 µg | ¥28,380 | |
600252 | 100 µg | ¥71,060 |
Gamma-aminobutyric acid, B [GABAB] receptors are located in the midbrain and help facilitate inhibitory signaling in the central nervous system. GABAB receptors exist as heterodimers, comprising of two subunits: GABABR1 and GABABR2 with 7 transmembrane domains, neither of which is fully functional when expressed alone. The GABABR1 inhibits neurotransmitter activity via binding γ-aminobutyric acid (GABA), following G protein-coupled pathway activation through interacting with GABABR2. Subsequently, this signaling cascade changes ion channel activity and induces adenylyl cyclase to interfere synaptic responses. A decrease in GABABR2 functional efficacy is implicated in several diseases, such as Rett syndrome and epileptic encephalopathy.
Product DetailsProduct Details
- Verified Reactivity
- Rat, Mouse, Human
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Immunogen
- Partial recombinant mouse GABABR2 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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WB - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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It is recommended to boil cell or tissue lysates at 70°C for western blot.
Reactivity of clone QA19A84 with human GABABR2 is observed but is weaker than reactivity with mouse or rat GABABR2. - RRID
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AB_2910469 (BioLegend Cat. No. 600251)
AB_2910469 (BioLegend Cat. No. 600252)
Antigen Details
- Structure
- GABABR2 is a 941 amino acid protein with a molecular mass of 106 kD.
- Distribution
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Membrane
- Function
- Synaptic Transmission
- Interaction
- GABABR1
- Biology Area
- Cell Biology, Neuroscience, Synaptic Biology
- Antigen References
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- Yoo Y, et al. 2017. Ann Neurol. 82:466-478.
- Duthey B, et al. 2002. J Biol Chem. 277:3236-41.
- Filippov A, et al. 2000. J Neurosci. 20:2867-74.
- Blein S, et al. 2000. Cell Mol Life Sci. 57:635-50.
- Gene ID
- 9568 View all products for this Gene ID
- UniProt
- View information about GABABR2 on UniProt.org
Other Formats
View All GABABR2 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-GABABR2 Recombinant Antibody | QA19A84 | WB |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-GABABR2 Recombinant Antibody
Total tissue lysates (15 µg total protein) from rat brain (L...
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