- Clone
- Basophil/2D7 (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![Basophilslash2D7_A647_122209 Basophilslash2D7_A647_122209](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/Basophilslash2D7_A647_12220.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood mononuclear cells surface stained with CCR3 PE and then intracellularly stained with Basophil/2D7 Alexa Fluor®647
The Basophil/2D7 mAb specifically recognizes the human basophil population. The antigen that this antibody binds is present in cell granules but its identity is still unknown.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Lysates of purified basophils
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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ICFC - Quality tested
ICC, IHC-P, IHC-F - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Additional reported applications for the relevant formats include: immunofluorescence staining7, and immunohistochemistry of acetone-fixed frozen sections1 and formalin-fixed paraffin-embedded sections1-6.
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Application References
(PubMed link indicates BioLegend citation) -
- Kepley CL, et al. 1995. J. Immunol. 154:6548. (IHC)
- Irani AM, et al. 1998. J. Allergy Clin. Immunol. 1998 101:354. (IHC)
- Agis H, et al. 2006. J. Clin. Pathol. 59:396. (IHC)
- Plager DA, et al. 2006. J. Allergy Clin. Immunol. 117:626. (IHC)
- Sperr WR, et al. 2001. Blood 98:2200. (IHC)
- Aichberger KJ, et al. 2006. Blood 108:3538. (IHC)
- Kepley CL, et al. 1998. J. Allergy Clin. Immun. 102:304. (IF)
- RRID
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AB_1967135 (BioLegend Cat. No. 346202)
Antigen Details
- Distribution
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Basophils.
- Cell Type
- Basophils
- Biology Area
- Immunology, Innate Immunity
- Gene ID
- NA
- UniProt
- View information about Basophil on UniProt.org
Related FAQs
Other Formats
View All Basophil Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human Basophils (2D7) | Basophil/2D7 | ICFC,ICC,IHC-P,IHC-F |
Alexa Fluor® 647 anti-human Basophils (2D7) | Basophil/2D7 | ICFC |
PE anti-human Basophils (2D7) | Basophil/2D7 | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human Basophils (2D7)
Human peripheral blood mononuclear cells surface stained wit... -
Alexa Fluor® 647 anti-human Basophils (2D7)
Human peripheral blood mononuclear cells surface stained wit... -
PE anti-human Basophils (2D7)
Human peripheral blood mononuclear cells were stained with C...
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