- Clone
- E11 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- III 204
- Other Names
- C3b/C4b receptor, complement receptor type 1 (CR1)
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![E11_Pure_060308 E11_Pure_060308](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/E11_Pure_060308.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
Human peripheral blood lymphocytes stained purified E11, followed by anti-mouse IgG FITC
CD35 is a type I single chain of glycoprotein, also known as C3b/C4b receptor, Complement Receptor type 1 or CR1. Four molecular weight allotypes (160kD, 190kD, 220kD, and 250kD) have been described. CD35 is expressed on granulocytes, monocytes, B cells, erythrocytes, and follicular dendritic cells, as well as subsets of NK and T cells. CD35 binds complement C3b, C4b, or iC3, and iC4, and plays important roles in both innate and adoptive immune response via mediating phagocytosis by granulocytes and monocytes. CD35 has also been reported to inhibit T-cell proliferation.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IP, IHC - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: spatial biology (IBEX)4,5.
-
Application References
(PubMed link indicates BioLegend citation) - Product Citations
-
- RRID
-
AB_1089032 (BioLegend Cat. No. 333402)
Antigen Details
- Structure
- Type I glycoprotein with four allotypes, 160kD, 190kD, 220kD, 250kD
- Distribution
-
Granulocytes, monocytes, B cells, erythrocytes, follicular dendritic cells, subsets of NK and T cells
- Function
- Adhesion, mediate phagocytosis, inhibit T-cell proliferation
- Ligand/Receptor
- C3b, iC3, C4b, iC4
- Cell Type
- B cells, Dendritic cells, Erythrocytes, Granulocytes, Monocytes, NK cells, T cells
- Biology Area
- Cell Biology, Complement, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
-
1. Zola Heddy, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules:The CD markers. WILEY-LISS
2. Klickstein LB, et al. 1988. J. Exp. Med. 168:1699 - Gene ID
- 1378 View all products for this Gene ID
- UniProt
- View information about CD35 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All CD35 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD35 | E11 | FC,IP,IHC |
FITC anti-human CD35 | E11 | FC |
PE anti-human CD35 | E11 | FC,SB |
TotalSeq™-A0167 anti-human CD35 | E11 | PG |
TotalSeq™-C0167 anti-human CD35 | E11 | PG |
TotalSeq™-B0167 anti-human CD35 | E11 | PG |
Biotin anti-human CD35 | E11 | FC |
Brilliant Violet 421™ anti-human CD35 | E11 | FC |
TotalSeq™-D0167 anti-human CD35 | E11 | PG |
APC anti-human CD35 | E11 | FC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-human CD35
Human peripheral blood lymphocytes stained purified E11, fol... -
FITC anti-human CD35
Human peripheral blood lymphocytes stained with E11 FITC -
PE anti-human CD35
Human peripheral blood lymphocytes stained with E11 PE Confocal image of human lymph node sample acquired using the... Confocal image of human lymph node sample acquired using the... -
TotalSeq™-A0167 anti-human CD35
-
TotalSeq™-C0167 anti-human CD35
-
TotalSeq™-B0167 anti-human CD35
-
Biotin anti-human CD35
Human peripheral blood lymphocytes were stained with anti-hu... -
Brilliant Violet 421™ anti-human CD35
Human peripheral blood lymphocytes were stained with anti-hu... -
TotalSeq™-D0167 anti-human CD35
-
APC anti-human CD35
Human peripheral blood lymphocytes were stained with anti-hu...
Follow Us