- Clone
- CBR-IC3/1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- ICAM-3
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![CBR-IC3slash1_Pure_090808.jpg CBR-IC3slash1_Pure_090808.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/CBR-IC3slash1_Pure_090808.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes stained with purified CBR-IC3/1, followed by anti-mouse IgG FITC
CD50 is a 120-140 kD type I membrane protein also known as intercellular adhesion molecule 3 (ICAM-3). It is broadly expressed on leukocytes, endothelial cells, and Langerhans cells. CD50 is a member of the Ig superfamily. It provides costimulatory signal for T cells and regulates cell adhesion through interaction with LFA-1 (CD11a/CD18, αL/β2) integrin.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Baboon, Cynomolgus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- SKW3 cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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FC - Quality tested
IP - Reported in the literature, not verified in house
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Additional reported applications include: immunoprecipitation1 and blocking1-3.
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Application References
(PubMed link indicates BioLegend citation) -
- deFougerolles AR and Springer TA, et al. 1992. J. Exp. Med. 175:185. (IP, Block)
- Geijtenbeek T, et al. 2000. Cell 100:575. (Block)
- Giannoni E, et al. 2003. J Biol. Chem. 278:36763. (Block)
- Product Citations
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- RRID
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AB_1186142 (BioLegend Cat. No. 330002)
Antigen Details
- Structure
- Ig superfamily, type I membrane protein, 130 kD
- Distribution
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Thymocytes, T and B cells, monocytes, granulocytes, endothelial cells
- Function
- Adhesion, costimulation
- Ligand/Receptor
- CD11a/CD18 (LFA-1), integrin αL/ß2
- Cell Type
- B cells, Endothelial cells, Granulocytes, Monocytes, T cells, Thymocytes
- Biology Area
- Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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1. Valent P, et al. 2001. Immunol. Rev. 179:74.
- Gene ID
- 3385 View all products for this Gene ID
- UniProt
- View information about CD50 on UniProt.org
Related FAQs
Other Formats
View All CD50 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD50 (ICAM-3) | CBR-IC3/1 | FC,IP |
PE anti-human CD50 (ICAM-3) | CBR-IC3/1 | FC |
APC anti-human CD50 (ICAM-3) | CBR-IC3/1 | FC |
PerCP/Cyanine5.5 anti-human CD50 (ICAM-3) | CBR-IC3/1 | FC |
PE/Cyanine7 anti-human CD50 (ICAM-3) | CBR-IC3/1 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD50 (ICAM-3)
Human peripheral blood lymphocytes stained with purified CBR... -
PE anti-human CD50 (ICAM-3)
Human peripheral blood lymphocytes stained with CBR-IC3/1 PE -
APC anti-human CD50 (ICAM-3)
Human peripheral blood lymphocytes stained with CBR-IC3/1 AP... -
PerCP/Cyanine5.5 anti-human CD50 (ICAM-3)
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Cyanine7 anti-human CD50 (ICAM-3)
Human peripheral blood lymphocytes were stained with anti-hu...
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