- Clone
- RPA-T8 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- IV T171
- Other Names
- T8, Leu2
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![RPA-T8_Purified_CD8a_CyTOF_112113 RPA-T8_Purified_CD8a_CyTOF_112113](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/RPA-T8_Purified_CD8a_CyTOF_112113.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 146Nd-anti-CD8a (RPA-T8). Data provided by DVS Sciences.
CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation, and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.
Product DetailsProduct Details
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- Chimpanzee, Baboon, Pigtailed Macaque, Sooty Mangabey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
CyTOF®, PG - Verified - Recommended Usage
-
This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Application Notes
-
The RPA-T8 antibody does not block the binding of HIT8a antibody to CD8a. Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed frozen sections3 and costimulation of T cell responses4. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 301073 & 301074).
- Additional Product Notes
-
Maxpar® is a registered trademark of Standard BioTools Inc.
-
Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
- Magidovich E, et al. 2007. P. Natl. Acad. Sci. USA 104:13022.
- Thakarl D, et al. 2008.J. immunol. 180:7431. PubMed
- Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
- Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Rout N, et al. 2010. PLoS One 5:e9787. (FC)
- Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
- Product Citations
-
- RRID
-
AB_2562810 (BioLegend Cat. No. 301053)
Antigen Details
- Structure
- Ig superfamily, homodimer or heterodimer with CD8β, 32-34 kD
- Distribution
-
Majority of thymocytes, T cell subset, NK cells
- Function
- MHC class I co-receptor, thymic differentiation, T cell activation
- Ligand/Receptor
- MHC Class I molecules
- Cell Type
- Dendritic cells, NK cells, T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
- Gene ID
- 925 View all products for this Gene ID
- UniProt
- View information about CD8alpha on UniProt.org
Related FAQs
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
-
We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
-
We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
-
We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
-
The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
Other Formats
View All CD8a Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
APC anti-human CD8a
Human peripheral blood lymphocytes stained with RPA-T8 APC -
APC/Cyanine7 anti-human CD8a
Human peripheral blood lymphocytes were stained with CD3 FIT... -
Biotin anti-human CD8a
Human peripheral blood lymphocytes stained with biotinylated... -
FITC anti-human CD8a
Human peripheral blood lymphocytes stained with RPA-T8 FITC -
PE anti-human CD8a
Human peripheral blood lymphocytes stained with RPA-T8 PE Human peripheral blood was stained with CD8a (clone RPA-T8) ... -
PE/Cyanine5 anti-human CD8a
-
PE/Cyanine7 anti-human CD8a
Human peripheral blood lymphocytes stained with RPA-T8 PE/Cy... -
Purified anti-human CD8a
Human peripheral blood lymphocytes stained with purified RPA... -
Alexa Fluor® 488 anti-human CD8a
Human peripheral blood lymphocytes stained with RPA-T8 Alexa... -
Alexa Fluor® 647 anti-human CD8a
Human peripheral blood lymphocytes stained with RPA-T8 Alexa... -
Pacific Blue™ anti-human CD8a
Human peripheral blood lymphocytes stained with RPA-T8 Pacif... -
Alexa Fluor® 700 anti-human CD8a
Human peripheral blood lymphocytes stained with RPA-T8 Alexa... -
PerCP anti-human CD8a
Human peripheral blood lymphocytes stained with RPA-T8 PerCP -
PerCP/Cyanine5.5 anti-human CD8a
Human peripheral blood lymphocytes were stained with CD3 APC... -
Brilliant Violet 421™ anti-human CD8a
Human peripheral blood mononuclear cells were fixed with 2% ... Human peripheral blood lymphocytes were stained with CD3 FIT... -
Brilliant Violet 570™ anti-human CD8a
Human peripheral blood lymphocytes were stained with CD3 and... -
Brilliant Violet 605™ anti-human CD8a
Human peripheral blood lymphocytes were stained with CD3 FIT... -
Brilliant Violet 650™ anti-human CD8a
Human peripheral blood lymphocytes were stained with CD3 FIT... -
Brilliant Violet 711™ anti-human CD8a
Human peripheral blood lymphocytes were stained with CD3 FIT... -
Brilliant Violet 785™ anti-human CD8a
Human peripheral blood lymphocytes were stained with CD3 FIT... -
Brilliant Violet 510™ anti-human CD8a
Human peripheral blood lymphocytes were stained with CD3 FIT... -
Purified anti-human CD8a (Maxpar® Ready)
Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 146Nd-an... -
Alexa Fluor® 594 anti-human CD8a
Human peripheral mononuclear cells were fixed with 2% parafo... Human peripheral blood lymphocytes were stained with CD8 (cl... -
PE/Dazzle™ 594 anti-human CD8a
Human peripheral blood lymphocytes were stained with CD8 (cl... -
APC/Fire™ 750 anti-human CD8a
Human peripheral blood lymphocytes were stained with CD3 FIT... -
TotalSeq™-A0080 anti-human CD8a
-
TotalSeq™-B0080 anti-human CD8a
-
TotalSeq™-C0080 anti-human CD8a
-
Ultra-LEAF™ Purified anti-human CD8a
Human peripheral blood lymphocytes stained with LEAF™ purifi... -
Spark Violet™ 423 anti-human CD8a Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark Red™ 718 anti-human CD8a
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark PLUS UV™ 395 anti-human CD8a
Human peripheral blood lymphocytes were stained with anti-hu...
Follow Us