- Clone
- Tü36 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- MHC class II, Major Histocompatibility complex II, human leukocyte antigen, HLA
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![Tu36_Purified_HLA-DR_Antibody_FC_012314.jpg Tu36_Purified_HLA-DR_Antibody_FC_012314.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/Tu36_Purified_HLA-DR_Antibody_FC_012314.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were stained with purified HLA-DR (clone Tü36) (filled histogram) or mouse IgG2b, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
HLA-DR is a heterodimeric cell surface glycoprotein comprised of an α (heavy) chain and a β (light) chain. They are expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells, and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. Variations in the HLA gene expression are crucial to graft survival.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Baboon, Cynomolgus, Cat, Dog, Cow
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human PBL
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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FC - Quality tested
IP, WB - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Additional reported applications (of relevant formats) includes Western blotting4, immunoprecipitation4, and in vitro blocking5. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (contact our custom solutions team).
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Application References
(PubMed link indicates BioLegend citation) -
- Pawelec G, et al. 1985. Hum. Immunol. 12:165. (FC)
- Shaw S, et al. 1985. Hum. Immunol. 12:191. (FC)
- Ziegler A, et al. 1986. Immunobiology. 171:77. (FC)
- Cebulla CM, et al. 2002. J. Immunol. 169:167. (WB, FC, IP)
- Khaw LT, et al. 2013. PLOS One. 8:e69521. (Block)
- Product Citations
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- RRID
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AB_2563138 (BioLegend Cat. No. 361602)
Antigen Details
- Structure
- Ig superfamily, MHC class II, heterodimeric transmembrane protein
- Distribution
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B cells, activated T cells, monocytes/macrophages, dendritic cells, other APCs
- Function
- Antigen presentation
- Ligand/Receptor
- CD3/TCR, CD4
- Cell Type
- Antigen-presenting cells, B cells, Dendritic cells, Macrophages, Monocytes, T cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
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1. Thorsby E. 1974. Transplant. Rev. 18:51.
2. Qvigstad E, et al. 1984. Hum. Immunol. 11:207.
3. Servenius B, et al. 1984. EMBO J. 3:3209.
4. Ottenhoff TH, et al. 1985. Hum. Immunol. 13:105.
5. Strassmann G, et al. 1985. Hum. Immunol. 13:125.
6. Trowsdale J, et al. 1985. Immunol. Rev. 85:5. - Gene ID
- 3122 View all products for this Gene ID 3123 View all products for this Gene ID 3113 View all products for this Gene ID 3115 View all products for this Gene ID
- UniProt
- View information about HLA-DR on UniProt.org
Related FAQs
Other Formats
View All HLA-DR Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human HLA-DR | Tü36 | FC,IP,WB |
PerCP/Cyanine5.5 anti-human HLA-DR | Tü36 | FC |
APC anti-human HLA-DR | Tü36 | FC |
FITC anti-human HLA-DR | Tü36 | FC |
PE anti-human HLA-DR | Tü36 | FC |
PE/Cyanine7 anti-human HLA-DR | Tü36 | FC |
APC/Fire™ 750 anti-human HLA-DR | Tü36 | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human HLA-DR
Human peripheral blood lymphocytes were stained with purifie... -
PerCP/Cyanine5.5 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with HLA-DR ... -
APC anti-human HLA-DR
Human peripheral blood lymphocytes were stained with HLA-DR ... -
FITC anti-human HLA-DR
Human peripheral blood lymphocytes were stained with purifie... -
PE anti-human HLA-DR
Human peripheral blood lymphocytes were stained with HLA-DR ... -
PE/Cyanine7 anti-human HLA-DR
Human peripheral blood monocytes were stained with HLA-DR (C... -
APC/Fire™ 750 anti-human HLA-DR
Human peripheral blood monocytes were stained with HLA-DR (C...
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