- Clone
- L24/18 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Inositol 1,4,5-trisphosphate receptor type 1, IP-3-R, IP3R 1, IP3 receptor, type 1 InsP3 receptor, inositol 1,4,5-triphosphate receptor 1, inositol 1,4,5-triphosphate receptor, type 1, type 1 inositol 1,4,5-trisphosphate receptor
- Previously
-
Covance Catalog# MMS-5151
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1_L24slash18_Purified_IP3R1_Antibody_IHC_030415 1_L24slash18_Purified_IP3R1_Antibody_IHC_030415](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/L24slash18_Purified_IP3R1_Antibody_IHC_030415.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
IHC staining of purified anti-IP3R1 antibody (clone L24/18) on formalin fixed paraffin embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. -
Western blot of purified anti-IP3R1 antibody (clone L24/18). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-IP3R1 antibody (clone L24/18) on formalin fixed paraffin embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-IP3R1 antibody (clone L24/18) on formalin fixed paraffin embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Inositol 1,4,5-triphosphate (IP3) is a calcium-regulating second messenger that receptor involved in ER stress-induced apoptosis. IP3 receptor, type 1 (IP3R1) is a receptor for IP3 expressed on the ER which opens a calcium channel on the ER after IP3 bidning. IP3R1 is expressed in developing and mature neurons and its dysregulation may be involved in progression of certain neurodegenerative diseases. IP3R1 is also involved in autophagy, cellular energenetics, macrophage inflammasomes, and other cellular processes.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against a fusion protein corresponding to amino acids 2680-2749 of rat IP3 receptor type 1.
- Formulation
- Phosphate-buffered solution.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/mL
- Storage & Handling
- This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
- Application
-
IHC-P - Quality tested
WB - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10 µg/mL is suggested. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody is less reactive in human samples than mouse and rat sample in WB.
-
Application References
(PubMed link indicates BioLegend citation) -
- Zhao G, et al. 2010. J. Gen. Physiol. 136(3):283-91 (WB)
- Product Citations
-
- RRID
-
AB_2564813 (BioLegend Cat. No. 817701)
Antigen Details
- Structure
- IP3R1 is a 2758 amino acid protein with an apparent molecular mass of 314 kD.
- Distribution
-
Cellular distribution: Plasma membrane, endoplasmic reticulum, and nucleus.
- Biology Area
- Cell Biology, Neuroscience, Synaptic Biology
- Molecular Family
- Postsynaptic proteins
- Antigen References
-
Tang T-S, et al. 2009. J. Neurosci. 29 (5) 1257-66.
- Gene ID
- 25262 View all products for this Gene ID
- UniProt
- View information about IP3R1 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All IP3R1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-IP3R1 | L24/18 | IHC-P,WB |
Biotin anti-IP3R1 | L24/18 | IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-IP3R1
IHC staining of purified anti-IP3R1 antibody (clone L24/18) ... Western blot of purified anti-IP3R1 antibody (clone L24/18).... IHC staining of purified anti-IP3R1 antibody (clone L24/18) ... IHC staining of purified anti-IP3R1 antibody (clone L24/18) ... -
Biotin anti-IP3R1
IHC staining of Biotin anti-IP3R1 antibody (Clone L24/18) on... IHC staining of Biotin anti-IP3R1 antibody (Clone L24/18) on...
Follow Us