- Clone
- W16064A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interferon regulatory factor 7 (IRF-7) protein
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![W16064A_PURE_IRF7_Antibody_041117 W16064A_PURE_IRF7_Antibody_041117](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W16064A_PURE_IRF7_Antibody_041117.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
Total cell lysates (15µg protein) from non-treated and recombinant mouse IFN-α treated (100nM, 24h) EL-4 cells were resolved by 4-20% Tris-glycine gel electrophoresis, transferred to nitrocellulose, and probed with 2µg/mL purified anti-IRF7 antibody (clone W16064A) antibody (upper) or loading control GAPDH (clone Poly6314) antibody (lower) . Proteins were visualized by chemiluminescence detection using a goat anti-rat-IgG secondary antibody conjugated to HRP for clone W16064A, and a donkey anti-rabbit IgG Antibody conjugated to HRP for GAPDH. Lane M is the MW ladder.
IRF-7 is a member of the interferon regulatory transcription factor family that plays key regulatory roles in innate immunity. Upon virus infection, IRF-7 forms a complex with MyD88 and TRAF6 when stimulated by toll-like receptors TLR7, TLR8, and TLR9, which in turn results in nuclear translocation and induction of interferon gene expression. IRF-7 gene expression is upregulated by the stimulation of interferon through the Jak-STAT signaling pathway, which forms a positive feedback loop. The protein level of IRF-7 after activation is then downregulated by the ubiquitin-proteasome system to avoid excessive production of interferons that may cause damage to the host. Virus induced type I interferon expression was completely abrogated in IRF-7 knockout mice, suggesting that IRF-7 is critical for interferon mediated host defense during virus infection.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Partial mouse IRF7 recombinant protein (127-487a.a.) expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 5.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
- Product Citations
-
- RRID
-
AB_2687056 (BioLegend Cat. No. 696402)
Antigen Details
- Structure
- 457 amino acids, predicted molecular weight of 51.2 kD, contains an IRF tryptophan pentad repeat responsible for DNA binding
- Distribution
-
Shuttles between the cytoplasm and nucleus; activated (phosphorylated) form of IRF-7 accumulates in the nucleus.
- Function
- Transcription factor that transactivates proinflammatory cytokines and type I interferon genes in response to virus infection.
- Interaction
- Forms homodimer or heterodimer with IRF-3, interacts with TICAM-1, TICAM-2, MyD88, and TRAF6.
- Biology Area
- Cell Biology, Transcription Factors
- Antigen References
-
1. Owens BM, et al. 2012. PLoS One. 7:e41050.
2. Yu Y, et al. 2010. Immunity. 33:863.
3. Liang Q, et al. 2011. J. Immunol. 186:1001.
4. Buss C, et al. 2010. J. Immunol. 184:3072.
5. Joo CH, et al. 2007. J. Virol. 81:8282.
6. Balachandran S, et al. 2007. J. Immunol. 178:2429. - Gene ID
- 54123 View all products for this Gene ID
- UniProt
- View information about IRF7 on UniProt.org
Other Formats
View All IRF7 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-IRF7 | W16064A | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-IRF7
Total cell lysates (15µg protein) from non-treated and recom...
Follow Us