- Clone
- QA17A53 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD40 ligand (CD40L), gp39, T-BAM, TRAP, Ly-62, TNFSF5
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![QA17A53_Purified_CD154_Antibody_1_022420 QA17A53_Purified_CD154_Antibody_1_022420](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/QA17A53_Purified_CD154_Antibody_1_022420.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Enriched C57BL/6 mouse splenic T cells were stimulated with PMA + ionomycin (Cell Activation Cocktail w/o Brefeldin A) for 4 hours then stained with anti-mouse CD4 APC and CD154 (clone QA17A53) purified followed by anti-rat IgG1, κ PE secondary antibody (left) or rat IgG1, κ purified isotype control followed by anti-rat IgG1 PE secondary antibody (right).
CD154 is a 39 kD TNF superfamily member also known as CD40 ligand, gp39, T-BAM, TRAP, and Ly-62. CD154 is an accessory molecule expressed predominantly on activated CD4+ lymphocytes that bind CD40. CD154 plays an important role in T-B cell costimulation.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Recombinant
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
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AB_2832561 (BioLegend Cat. No. 158102)
Antigen Details
- Structure
- TNF superfamily, 39kD
- Distribution
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Activated CD4+ T-cells
- Function
- T-B cell costimulation
- Interaction
- T-cells, Tregs
- Ligand/Receptor
- CD40
- Cell Type
- T cells, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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- Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
- Noelle RJ, et al. 1992. Proc. Natl. Acad. Sci. USA 89:6550.
- Banchereau J, et al. 1994. Annu. Rev. Immunol. 12:881.
- Clark EA and Ledbetter JA. 1986. Proc. Natl. Acad. Sci. USA 83:4494.
- Gene ID
- 21947 View all products for this Gene ID
- UniProt
- View information about CD154 on UniProt.org
Other Formats
View All CD154 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
PE anti-mouse CD154 Recombinant Antibody | QA17A53 | FC |
Purified anti-mouse CD154 Recombinant Antibody | QA17A53 | FC |
PE/Cyanine5 anti-mouse CD154 Recombinant Antibody | QA17A53 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-mouse CD154 Recombinant Antibody
Enriched C57BL/6 mouse splenic T cells were stimulated with ... -
Purified anti-mouse CD154 Recombinant Antibody
Enriched C57BL/6 mouse splenic T cells were stimulated with ... -
PE/Cyanine5 anti-mouse CD154 Recombinant Antibody
Enriched C57BL/6 mouse splenic T cells were stimulated with ...
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