- Clone
- SA271G2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Ms4a1, Ly-44
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![SA271G2_PURE_CD20_Antibody_1_100516 SA271G2_PURE_CD20_Antibody_1_100516](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/SA271G2_PURE_CD20_Antibody_1_100516.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were stained with CD45R/B220 APC and purified CD20 (clone SA271G2, top) or purified rat IgG2b, κ isotype control (bottom), followed by anti-rat IgG PE. -
CD20 is a 33-37 kD protein, a member of the MS4A family, with four transmembrane spanning regions that present as a homo-oligomeric complexes in the cell surface when associating with MHC class I and II, CD53, CD81, and CD82. CD20 is expressed on B cells and a subset of T cells, but not on plasma cells. CD20 regulates B-cell activation and proliferation. Its ligation promotes transmembrane Ca2+ trafficking. CD20 is an important therapeutic target in the treatment of B cell lymphomas and leukemias.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse CD20 transfected cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
Depletion - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
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Application References
(PubMed link indicates BioLegend citation) -
- Bongard, A, et al. 2019. PLoS Pathog. 15(9):e1008043. (Depletion) Pubmed
- RRID
-
AB_2632610 (BioLegend Cat. No. 152102)
Antigen Details
- Structure
- Member of the MS4A family, four transmembrane spanning regions, three isoforms of 37, 35, and 37 kD, depending on the phosphorylation degree.
- Distribution
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B cells and a subset of T cells
- Function
- Regulates B-cell activation and proliferation
- Interaction
- Associates with MHC class I and II, CD53, CD81, and CD82
- Cell Type
- B cells, T cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
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1. Morsy DE, et al. 2013. J. Immunol. 191:3112.
2. Lund FE, Randall TD. 2010. Nat. Rev. Immunol. 10:236.
3.Beers SA, et al. 2010. Blood 115:5191.
4. Kuijpers TW, et al. 2010. J. Clin. Invest. 120:214. - Gene ID
- 12482 View all products for this Gene ID
- UniProt
- View information about CD20 on UniProt.org
Other Formats
View All CD20 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD20 | SA271G2 | FC,Depletion |
Ultra-LEAF™ Purified anti-mouse CD20 | SA271G2 | FC,Depletion |
PE anti-mouse CD20 | SA271G2 | FC |
APC anti-mouse CD20 | SA271G2 | FC |
PE/Dazzle™ 594 anti-mouse CD20 Antibody | SA271G2 | FC |
FITC anti-mouse CD20 | SA271G2 | FC |
PE/Cyanine5 anti-mouse CD20 | SA271G2 | FC |
APC/Fire™ 750 anti-mouse CD20 | SA271G2 | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD20
C57BL/6 mouse splenocytes were stained with CD45R/B220 APC a... -
Ultra-LEAF™ Purified anti-mouse CD20
C57BL/6J mice were injected i.v. with 250 µg of Ultra-LEAF™ ... C57BL/6 mice were injected intravenously with 250 μg of Ultr... -
PE anti-mouse CD20
C57BL/6 mouse splenocytes were stained with CD45R/B220 APC/C... -
APC anti-mouse CD20
C57BL/6 mouse splenocytes were stained with CD45R/B220 APC/C... -
PE/Dazzle™ 594 anti-mouse CD20 Antibody
C57BL/6 mouse splenocytes were stained with CD45R/B220 APC a... -
FITC anti-mouse CD20
C57BL/6 mouse splenocytes were stained with CD45R/B220 APC/C... -
PE/Cyanine5 anti-mouse CD20
C57BL/6 mouse splenocytes were stained with anti-mouse CD45R... -
APC/Fire™ 750 anti-mouse CD20
C57BL/6 mouse splenocytes were stained with anti-mouse CD45R...
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