- Clone
- W19424P (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Tie-2, TEK
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![W19424P_PURE_CD202b_Antibody_022422 W19424P_PURE_CD202b_Antibody_022422](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W19424P_PURE_CD202b_Antibody_022422.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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bEnd.3 cells were stained with purified anti-mouse CD202b (Tie-2, CD202) (clone W19424P) (filled histogram) or rat IgG2b, κ isotype control (open histogram) followed by PE anti-rat IgG. -
C57 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 1 hour at room temperature. Then the section was stained with 10 µg/mL of purified anti-mouse CD202b (Tie-2, CD202) (clone W19424P) at 4°C overnight. On the next day, tissue was incubated with Alexa Fluor® 594 goat anti-rat IgG (clone Poly4054) (red) and anti-mouse CD3ε (clone 500A2) Alexa Fluor® 647 (green) for an hour at room temperature. Nuclei were counter-stained with DAPI (blue). The image was captured with a 10X object. -
C57 mouse frozen kidney section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 1 hour at room temperature. Then the section was stained with 10 µg/mL of purified anti-mouse CD202b (Tie-2, CD202) (clone W19424P) at 4°C overnight. On the next day, tissue was incubated with Alexa Fluor® 594 goat anti-rat IgG (clone Poly4054) (red) for an hour at room temperature. Nuclei were counter-stained with DAPI (blue). The image was captured with a 10X object.
CD202b, also known as Tie-2 or TEK, is a 145 kD type I transmembrane protein. It is a member of the receptor tyrosine kinase (RTK) family of proteins and is expressed by endothelial cells and their progenitors, quiescent hematopoietic stem cells (HSCs), and a subpopulation of monocytes. Angiopoietin-1 (Ang-1) is an activator of CD202b to promote, maintain, and stabilize mature vessels and to maintain HSCs in the quiescent state. Ang-2 is another ligand of CD202b, which is involved in postnatal angiogenesis and in antagonizing the effects of Ang-1. Tie-2 also binds to Ang-4.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2922458 (BioLegend Cat. No. 110402)
Antigen Details
- Structure
- The receptor tyrosine kinase (RTK) family
- Distribution
-
Endothelial cells and their progenitors, quiescent hematopoietic stem cells (HSCs), and a subpopulation of monocytes
- Cell Type
- Endothelial cells, Epithelial cells, T cells
- Biology Area
- Angiogenesis, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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- Fathers KE, et al. 2005. Am. J. Pathol. 167:1753-62.
- Yano M, et al. 1997. Blood 89:4317-26.
- Kolatsi-Joannou M, et al. 2001. Dev. Dyn. 222:120-6.
- Gene ID
- 21687 View all products for this Gene ID
- UniProt
- View information about CD202b on UniProt.org
Related FAQs
Other Formats
View All CD202b Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD202b (Tie-2, CD202) | W19424P | FC,IHC-F |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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