- Clone
- W17001A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Eomesodermin, T-box brain protein 2 (TBR-2)
- Isotype
- Rat IgG2b, λ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![W17001A_PURE_EOMES_Antibody_1_081420 W17001A_PURE_EOMES_Antibody_1_081420](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W17001A_PURE_EOMES_Antibody_1_081420.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were surface stained with CD8a Alexa Fluor® 488 then treated with True-Nuclear Transcription Factor Buffer Set. Cells were then stained with purified EOMES (clone W17001A) (left) or purified rat IgG2b, λ isotype control (right) followed by anti-rat IgG Alexa Fluor® 647. -
Whole cell extracts (15 µg total protein) from NK-92 (human positive control), HeLa (human negative control), C57/BL6 primary splenocytes (mouse positive control), and NIH/3T3 (mouse negative control) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-mouse EOMES antibody (clone W17001A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker. -
Whole cell extracts (250 µg total protein) prepared from NK-92 cells were immunoprecipitated overnight with 2.5 µg of purified rat IgG2b, κ isotype ctrl antibody (Cat. No. 400602) or purified anti-mouse EOMES antibody (clone W17001A). The resulting IP fractions and whole cell extract input (10%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with W17001A. Lane M: Molecular weight marker. -
IHC staining of purified anti-mouse EOMES (clone W17001A) on formalin-fixed paraffin-embedded human colon tumor tissue. Following antigen retrieval using Tris-EDTA buffer (10 mM Tris, 1 mM EDTA, pH 9.0), the tissue was incubated without (panel A) and with (panel B) 5 µg/mL of primary antibody followed by incubation with Alexa Fluor ® 647 goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 µm
Eomesodermin, or EOMES, is a transcription factor in the T-box family. During embryonic development, EOMES is crucial in regulating trophoblast differentiation, gastrulation, mesoderm delamination, and development of the cerebral cortex. In the immune system, EOMES is involved in the activation, migration, and differentiation of CD8+ T cells. In cooperation with another T-box transcription factor, T-bet, EOMES induces production of IFN-γ and enhances cytotoxic activities of effector CD8+ T cells. EOMES has been shown to be required to maintain long-term memory of CD8+ T cells and is important for homeostasis of memory and effector T cells.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse EOMES recombinant protein (463-707 a.a.) expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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ICFC - Quality tested
WB, IP, IHC-P - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. For western blotting, the suggested use of this reagent is 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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W17001A was tested for ICC using 4% PFA-fixed HeLa (negative control) and NK-92 (positive control cells) permeabilized with either Triton X-100 or methanol. Both methods resulted in high non-specific staining in HeLa cells. We therefore don’t recommend W17001A for this application.
- RRID
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AB_2876538 (BioLegend Cat. No. 157702)
Antigen Details
- Distribution
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Nucleus
- Antigen References
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- Cooper L, et al. 2018. Plos One. 13(12).
- Pikovskaya O, et al. 2016. J.Immunol. 196(4):1449-54.
- Lupar E, et al. 2015. J.Immunol. 195(10):4742-52.
- Gene ID
- 13813 View all products for this Gene ID
- UniProt
- View information about EOMES on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All EOMES Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Alexa Fluor® 647 anti-mouse EOMES | W17001A | ICFC |
Purified anti-mouse EOMES | W17001A | ICFC,WB,IP,IHC-P |
PE anti-mouse EOMES Antibody | W17001A | ICFC |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Alexa Fluor® 647 anti-mouse EOMES
C57BL/6 mouse splenocytes were surface stained with CD8a Ale... -
Purified anti-mouse EOMES
C57BL/6 mouse splenocytes were surface stained with CD8a Ale... Whole cell extracts (15 µg total protein) from NK-92 (human ... Whole cell extracts (250 µg total protein) prepared from NK-... IHC staining of purified anti-mouse EOMES (clone W17001A) on... -
PE anti-mouse EOMES Antibody
C57BL/6 mouse splenocytes were stained with anti-mouse CD8a ...
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