- Clone
- RMK-45 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Immunoglobulin light chain kappa
- Isotype
- Rat IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![RMK-45_Purified_Igk_Antibody_FC_1_041714 RMK-45_Purified_Igk_Antibody_FC_1_041714](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/RMK-45_Purified_Igk_Antibody_FC_1_041714.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 splenocytes were stained with purified anti-mouse light chain Ig κ (clone RMK-45) (filled histogram) or purified rat IgG1, κ isotype control, followed by anti-rat IgG PE (opened histogram). -
Human peripheral blood lymphocytes were stained with purified CD3 (clone UCHT1), and then stained with purified anti-mouse Ig light chain κ (clone RMK-45) (filled histogram) or purified rat IgG1, κ isotype control, followed by anti-rat IgG PE (opened histogram).
The RMK-45 monoclonal antibody reacts with immunoglobulin light chain kappa in all tested mouse haplotype (Igh-a and b). It does not react with the lambda chain.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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FC - Quality tested
ELISA - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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The RMK-12 monoclonal antibody may be used as a primary antibody for ELISA or as secondary for detection of mouse Ig kappa.
- Product Citations
-
- RRID
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AB_2563297 (BioLegend Cat. No. 409502)
Antigen Details
- Structure
- Immunoglobulin family
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Protein Synthesis
- Antigen References
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1. Ludwig TE, et al. 2006. Nat. Methods. 3:637.
2. Nguyen DH, et al. 2005. J. Immunol. 175:228.
3. Bardor M, et al. 2005. J. Biol. Chem. 280:4228.
4. Diaz SL, et al. 2009. PLoS One. 4:e4241. - Gene ID
- 243469 View all products for this Gene ID
- UniProt
- View information about Ig light chain kappa on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All Ig light chain κ Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse Ig light chain κ | RMK-45 | FC,ELISA |
APC/Cyanine7 anti-mouse Ig light chain κ | RMK-45 | FC |
PE anti-mouse Ig light chain κ | RMK-45 | FC |
Alexa Fluor® 700 anti-mouse Ig light chain κ | RMK-45 | FC |
FITC anti-mouse Ig light chain κ | RMK-45 | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse Ig light chain κ
C57BL/6 splenocytes were stained with purified anti-mouse li... Human peripheral blood lymphocytes were stained with purifie... -
APC/Cyanine7 anti-mouse Ig light chain κ
C57BL/6 splenocytes were stained with anti-mouse Ig light ch... Human peripheral blood lymphocytes were stained with (filled... -
PE anti-mouse Ig light chain κ
C57BL/6 splenocytes were stained with anti-mouse Ig light ch... Human peripheral blood lymphocytes were stained with (filled... -
Alexa Fluor® 700 anti-mouse Ig light chain κ
C57BL/6 splenocytes were stained with anti-mouse Ig light ch... Human peripheral blood lymphocytes were stained with (filled... -
FITC anti-mouse Ig light chain κ
C57BL/6 splenocytes were stained with anti-mouse Ig light ch... Human peripheral blood lymphocytes were stained with (filled...
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