- Clone
- MA-69 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Igh-6a
- Isotype
- Mouse (C57BL/6) IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![DS1_Pure_122007 DS1_Pure_122007](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/DS1_Pure_122007.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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BALB/c mouse splenocytes stained with purified MA-69, followed by anti-mouse IgG1 FITC
This antibody was raised by immunization with IgMa mAb TC31. It specifically reacts with mouse IgM of Igh-Ca and the related haplotypes (c, f, g, h, and j). It has been reported that beads coated with MA-69 antibody can stimulate B cell proliferation.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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FC - Quality tested
IHC, ELISA - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Additional reported applications (for the relevant formats) include: ELISA as capture antibody or detection antibody, stimulation of B cell proliferation, and immunohistochemistry of acetone-fixed frozen sections.
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Application References
(PubMed link indicates BioLegend citation) -
- Sieckmann D, et al. 1991. Hybridoma 10:121.
- Stall AM. 1996. Weir's Handbook of Experimental Immunology pp. 27.
- Product Citations
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- RRID
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AB_940549 (BioLegend Cat. No. 408602)
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All IgMa Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Biotin anti-mouse IgMa | MA-69 | ELISA Detection,FC,IHC |
FITC anti-mouse IgMa | MA-69 | FC |
PE anti-mouse IgMa | MA-69 | FC |
Purified anti-mouse IgMa | MA-69 | FC,IHC,ELISA |
PerCP/Cyanine5.5 anti-mouse IgMa | MA-69 | FC |
APC anti-mouse IgMa | MA-69 | FC |
PE/Cyanine7 anti-mouse IgMa | MA-69 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-mouse IgMa
BALB/c splenocytes stained with MA-69 Biotin, followed by Sa... -
FITC anti-mouse IgMa
Balb/c (upper) and C57BL/6 (bottom) splenocytes stained with... -
PE anti-mouse IgMa
Balb/c mouse splenocytes stained with MA-69 PE -
Purified anti-mouse IgMa
BALB/c mouse splenocytes stained with purified MA-69, follow... -
PerCP/Cyanine5.5 anti-mouse IgMa
BALB/c mouse spenocytes were stained with PerCP/Cyanine5.5 I... -
APC anti-mouse IgMa
BALB/c mouse spenocytes were stained with APC IgMa (clone MA... -
PE/Cyanine7 anti-mouse IgMa
BALB/c mouse spenocytes were stained with PE/Cyanine7 IgM
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