- Clone
- W16082A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- DNA-binding factor KBF2, H2TF1, Lymphocyte translocation chromosome 10 protein, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2, Oncogene Lyt-10
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Total cell lysates (15 µg total protein) from A431, HaCat, THP-1, and HeLa cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-NF-κB p100/p52 Antibody, clone W16082A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control at a 1:5000 dilution (lower). Lane M: Molecular Weight marker. -
Total cell lysates (15 µg protein) from THP-1, HeLa and NIH3T3 were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 1µg/mL purified anti- NF-κB2 antibody (clone W16082A) antibody (upper). Proteins were visualized using a goat anti-rat-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower). Lane M is the molecular weight ladder. -
HeLa cells were fixed with 4% paraformaldehyde (PFA) for fifteen minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 0.0 and 0.5 µg/mL anti- NF-κB2 antibody (clone W16082A) overnight at 4°C followed by Alexa Fluor™ 594 (red) conjugated goat anti-rat IgG for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
Nuclear factor NF-kappa-B p100/p52 subunit (NF-κB2) is a member of the Rel/NF-κB family of transcription factors. NF-κB2 p100 subunit contains an ankyrin repeat domain that has IκB activity to retain NF-κB2 proteins in the cytoplasm. Upon activation, NF-κB2 p100 subunit is processed to p52 subunit through the ubiquitin-proteasome pathway and translocated to the nucleus. NF-κB2 p52 subunit forms homo- or heterodimers with other Rel-like domain-containing proteins, including c-REL, RELA/p65, RELB, NFKB1/p105, and NFKB1/p50, subsequently binds to specific kappa-B sites and regulates expression of their target genes. NF-κB participates in the regulation of peripheral lymphoid development, thymic development, and B cell maturation. Mutations in the NF-κB2 gene lead to defects in B cell development and have been associated with lymphoma and other hematogenous malignancies.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Human NF-κB2 recombinant protein (1-250 a.a.) expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/ml. For immunocytochemistry, a concentration range of 0.2 - 1.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone W16082A does not cross-react with mouse (in-house tested).
- RRID
-
AB_2650935 (BioLegend Cat. No. 695902)
Antigen Details
- Structure
- NF-κB2 p100 subunit contains 900 amino acids with a predicted molecular weight of 96.7 kD. Contains an N-terminal RHD domain for DNA binding and a C-terminal ankyrin repeat domain. NF-κB2 p100 subunit is processed to a p52 subunit upon activation.
- Distribution
-
Nucleus and cytoplasm.
- Function
- NF-κB2 is a transcription factor involved in the regulation of peripheral lymphoid development, thymic development, and B cell maturation.
- Interaction
- Forms homodimers or heterodimers with other Rel-like domain-containing proteins including c-REL, RELA/p65, RELB, NFKB1/p105, and NFκB1/p50. Interacts with BCL3, BTRC, TSC22D3, and MAP3K8.
- Biology Area
- Cell Biology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Lee CE, et al. 2014. Blood. 124:2964.
2. Savinova OV, et al. 2009. Mol Cell. 34:591.
3. Tucker E, et al. 2007. J. Immunol. 179:7514.
4. Kim KE, et al. 2000. Oncogene. 19:1334.
5. Heusch M, et al. 1999. Oncogene. 18:6201.
6. de Wit H, et al. 1998. Leukemia. 12:363. - Gene ID
- 4791 View all products for this Gene ID
- UniProt
- View information about NF-kappaB p100/p52 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All NF-κB p100/p52 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-NF-κB p100/p52 | W16082A | WB,ICC |
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