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Purified anti-p63 (ΔN) Antibody

Pricing & Availability
Clone
Poly6190 (See other available formats)
Regulatory Status
RUO
Other Names
p63, δN p63-α, -β, -γ
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly6190_p63-deltaN_092811
Non-transfected 293 cell lysates (Lane 1) and p63 (deltaN) transfected 293 cell lysates (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-p63 (deltaN) antibody (clone Poly6190). Proteins were visualized using a donkey anti-rabbit-IgG secondary conjugated to HRP and chemiluminescence detection.
  • Poly6190_p63-deltaN_092811
    Non-transfected 293 cell lysates (Lane 1) and p63 (deltaN) transfected 293 cell lysates (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-p63 (deltaN) antibody (clone Poly6190). Proteins were visualized using a donkey anti-rabbit-IgG secondary conjugated to HRP and chemiluminescence detection.
Cat # Size Price Save
619001 50 µl (5 Western blots) ¥29,400
619002 200 µl (20 Western blots) ¥82,200
Description

p63 (ΔN) is a member of the p53 family. This protein has multiple isoforms TA p63-α, -β, γ, ΔN p63-α, -β, and -γ. The molecular weights of the p63 isoforms α=66 kD, β=52 kD, and γ=45 kD. This nuclear protein is essential for limb formation, epidermal morphogenesis, and epithelial stem cell regeneration. ΔN isoforms are antagonistic to p53; TA isoforms can transactivate p53 targets. The p63 ΔN isoforms repress TA isoforms; mutant p53 abrogates p63 transactivation. This protein interacts with p53, p73, and the various TA- and ΔN isoforms. The Poly6190 antibody recognizes human p63 ΔN isoforms and has been shown to be useful for Western blotting.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Modified peptide
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation
The antibody was purified by antigen-affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, store frozen at -20°C.
Application

WB - Quality tested
IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Medawar A, et al. 2008. PLoS ONE 3:e3441. PubMed
  2. Di Girolamo N, et al. 2009. Transplantation. 87:1571. PubMed
  3. Bray LJ, et al. 2012. Biomaterials. 33:3529. PubMed
  4. Zhou Y, et al. 2011. Clin. Invest. Med. 34:E184. (IHC)
  5. Stewart RM, et al. 2015. Invest Ophthalmol Vis Sci. 56:2021. PubMed
  6. Curtis KM, et al. 2015. PLoS One. 10:123642. PubMed
  7. Ahronian LG, et al. 2015. PLoS One.10:123816. PubMed
Product Citations
  1. Marsh T, et al. 2020. Dev Cell. 591:52. PubMed
  2. Hall EG, et al. 2020. Hum Mol Genet. 29:845. PubMed
  3. Bohnenpoll T, et al. 2016. J Am Soc Nephrol. 2016080849. PubMed
  4. Curtis K, et al. 2015. PLoS One. 10:123642. PubMed
  5. Larsen SB, et al. 2021. Cell Stem Cell. 28:1758. PubMed
  6. Bohnenpoll T, et al. 2017. PLoS Genet.. 10.1371/journal.pgen.1006951. PubMed
  7. Lin-Shiao E, et al. 2018. Genes Dev. 8:9905. PubMed
  8. Zhang B, et al. 2020. Nat Commun. 1.150694444. PubMed
  9. Bray L, et al. 2012. Biomaterials. 33:3529. PubMed
  10. Hazawa M, et al. 2018. EMBO Rep. 0.842361111. PubMed
  11. Wang Z et al. 2018. The Journal of cell biology. 217(6):1941-1955 . PubMed
  12. Li Y, et al. 2017. Mol Cancer Res.. 10.1158/1541-7786.MCR-17-0349. PubMed
  13. Geyer FC, et al. 2018. Nat Commun. 7:2013. PubMed
  14. Mamo TM, et al. 2017. Hum Mol Genet. 26:3553. PubMed
  15. Li M, et al. 2021. Nat Commun. 12:420. PubMed
  16. Traver G, et al. 2017. Free Radic Biol Med. 112:578. PubMed
  17. D'Amico S, et al. 2018. Genes Dev. 32:1175. PubMed
  18. Christians A, et al. 2020. Hum Mol Genet. 1192:29. PubMed
  19. Medawar A, et al. 2008. PLoS One. 2.514583333. PubMed
  20. Kim JE, et al. 2020. Nat Commun. 0.690277778. PubMed
  21. Abbas HA, et al. 2018. Cancer Res. 78:451. PubMed
  22. Zhuang L, et al. 2021. Cells. 10:. PubMed
  23. Rode I, et al. 2015. J Immunol. 195: 5678 - 5687. PubMed
  24. Crowley L, et al. 2020. Elife. 9:00. PubMed
  25. Böttinger P, et al. 2020. Carcinogenesis. 1605:41. PubMed
  26. Lu Y, et al. 2018. Stem Cells. 36:1875. PubMed
  27. Crowell PD et al. 2019. Cell Rep. 28(6):1499-1510 . PubMed
  28. Langa P, et al. 2018. J Dermatol Sci. 89:272. PubMed
  29. Meuser M, et al. 2022. Development. 149:. PubMed
  30. Yuan T, et al. 2020. Front Pharmacol. 11:120. PubMed
  31. Portal C, et al. 2022. Invest Ophthalmol Vis Sci. 63:3. PubMed
  32. Ruiz EJ, et al. 2021. Elife. 10:. PubMed
  33. Wu HH, et al. 2021. Nucleic Acids Res. 49:2740. PubMed
  34. Ahronian L, et al. 2015. PLoS One. 10:123816. PubMed
  35. Chen HJ, et al. 2019. J Exp Med. 216:674. PubMed
  36. Volckaert T et al. 2017. Developmental cell. 43(1):48-59 . PubMed
  37. Aydoğdu N, et al. 2018. Development. 145. PubMed
  38. Stewart R, et al. 2015. Invest Ophthalmol Vis Sci . 56:2021. PubMed
  39. Li M, et al. 2021. Signal Transduct Target Ther. 6:05. PubMed
  40. Masterton S, et al. 2019. Biores Open Access. 8:74. PubMed
  41. Hegde GV et al. 2019. Elife. 8 pii: e46551. PubMed
  42. Van Sciver N, et al. 2021. PLoS Pathog. 17:e1010045. PubMed
  43. Uchtmann KS, et al. 2020. Reprod Toxicol. 91:131. PubMed
  44. Chen M, et al. 2019. Cell Stem Cell. 25:501. PubMed
  45. Assmus AM, et al. 2018. Am J Physiol Renal Physiol. 314:F820. PubMed
  46. Napoli M, et al. 2022. Nat Commun. 13:614. PubMed
  47. Karsli Uzunbas G, et al. 2019. J Biol Chem. 10.1074/jbc.RA119.007965. PubMed
  48. Mao X, et al. 2022. Int J Mol Sci. 23:. PubMed
RRID
AB_2256361 (BioLegend Cat. No. 619001)
AB_2207170 (BioLegend Cat. No. 619002)

Antigen Details

Structure
p53 family, six isoforms, ΔN p63α, β, γ, approximately 66 kD, 52 kD, 45 kD
Distribution

Nuclear

Function
Essential for limb formation, epidermal morphogenesis, epithelial stem cell regeneration. ΔN isoforms antagonistic to p53; TA isoforms can transactivate p53 targets
Interaction
p53, p73, TA- and ΔN isoforms
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Transcription Factors
Antigen References

1. Yang A, et al. 1998. Mol Cell. 2:305.
2. Celli J, et al. 1999. Cell. 99:143.
3. Gaiddon C, et al. 2001. Mol Cell Biol. 21:1874.
4. Benard J, et al. 2003. Hum Mutat. 21:182.

Regulation
ΔN isoforms repress TA isoforms, mutant p53 abrogates p63 transactivation
Gene ID
8626 View all products for this Gene ID
UniProt
View information about p63 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 09/22/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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