- Clone
- M380B (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Mouse IgG1
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![M380A_PURE_Phosphothreonine_Antibody_1_121720.png M380A_PURE_Phosphothreonine_Antibody_1_121720.png](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/M380A_PURE_Phosphothreonine_Antibody_1_121720.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Whole cell lysates (15 μg) from untreated (-) or Calyculin A treated (+) (50 nM for 30 min) HeLa cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with 1.0 μg/mL (1:500 dilution) purified anti-Phosphoserine antibody (clone M380B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker. -
HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.5% Triton-X for 10 minutes, and blocked with 5% FBS for 30 minutes. Cells were then intracellularly stained with a 5.0 µg/mL (1:100 dilution) (panel A) purified anti-Phosphoserine antibody (clone M380B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG antibody (Cat. No. 405326) at 2.5 µg/mL. To demonstrate phospho-specificity of M380B, fixed and permeabilized HeLa cells were pre-treated with lambda protein phosphatase as a control prior to staining with M380B (panel B). Nuclei were counterstained with DAPI, and the image was captured with a 60X objective. -
Whole cell extracts (250 µg total protein) prepared from HeLa cells untreated (-) or treated (+) with Calyculin A (50 nM, 30 min) were immunoprecipitated overnight with 2.5 µg purified mouse IgG1, κ isotype ctrl antibody (Cat. No. 401401) or purified anti-Phosphoserine antibody (clone M380B). The resulting IP fractions and whole cell extract input (10%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with purified anti-Phosphoserine antibody (clone M380B). Lane M: Molecular weight marker. -
PBMCs treated with (positive control, filled histogram) or without (negative control, open histogram) Cell Activation Cocktail (without Brefeldin A) (Cat No. 423301) were fixed and permeabilized using True Phos™ Perm Buffer (Cat No. 425401) and intracellularly stained with Purified anti-Phosphoserine (clone M380B) or Purified anti-Mouse IgG1, κ isotype control (Cat No. 400101) (open histogram, dashed line) (representative for untreated and treated PBMCs), followed by Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322).
Cat # | Size | Price | Save |
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944101 | 25 µg | ¥25,960 | |
944102 | 100 µg | ¥64,460 |
Phosphorylation of specific serine residues is a post-translational modification event critical for regulating the activity of many proteins. Protein phosphorylation events can alter protein function through a variety of molecular mechanisms, ranging from conformational changes that can stimulate or inhibit protein function to facilitating protein-protein interactions with binding partners. Antibodies that can detect phosphoserine residues are valuable tools for identifying novel phospho-sites and characterizing changes in the post-translational state of a broad range of phosphorylated proteins.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat, All Species
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide containing phosphorylated serine
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, IP, ICFC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. For flow cytometric staining using our True-Phos™ Perm Buffer, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2890869 (BioLegend Cat. No. 944101)
AB_2890869 (BioLegend Cat. No. 944102)
Antigen Details
- Function
- Cell signaling
- Biology Area
- Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Phospho-Proteins, Protein Kinases/Phosphatase
- Antigen References
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1. Rogerson D. et. al. 2015. Nat. Chem. Biol. 11:496-503.
2. Steinfield J. et. al. 2014. ACS Chem. Biol. 9(5):1104-1112. - Gene ID
- NA
- UniProt
- View information about Phosphoserine on UniProt.org
Related FAQs
Other Formats
View All Phosphoserine Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Phosphoserine | M380B | WB,ICC,IP,ICFC |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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