- Clone
- N133/21 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Regulator of G-protein signaling 14, RAP1/RAP2-interacting protein
- Previously
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Covance Catalog# MMS-5248
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![N133.21_Purified_RGS14_IHC_020315.jpg N133.21_Purified_RGS14_IHC_020315.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/N133.21_Purified_RGS14_IHC_020315.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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IHC staining of anti-RGS14 antibody (clone N133/21) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with the primary antibody at 10 µg/mL overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species) was used for detection followed by hematoxylin and bluing solution counterstaining, according to the protocol provided. -
IHC staining of anti-RGS14 antibody (clone N133/21) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with the primary antibody at 10 µg/mL overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species) was used for detection followed by hematoxylin and bluing solution counterstaining, according to the protocol provided. -
Western blot of anti-RGS14 antibody (clone N133/21). Lane 1: molecular weight marker; Lane 2: 20µg of mouse brain lysate; Lane 3: 20µg of rat brain lysate. Blots were incubated with 10 µg/mL of primary antibody overnight at 4°C, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. Enhanced chemiluminescence was used as the detection system.
Regulator of G protein Signaling 14 (RGS14) modulates G-protein signaling by binding to G alpha subunits bound to GTP, converting GDP to GTP, and allowing G alpha subunits to bind to G beta/gamma subunits, which are inactive as a G protein.
Product DetailsProduct Details
- Verified Reactivity
- Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against a fusion protein corresponding to amino acids 1-544 (full-length) of rat RGS14.
- Formulation
- Phosphate-buffered solution.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/ml
- Storage & Handling
- This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
- Application
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IHC-P - Quality tested
WB - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by immunohistochemical staining.
The optimal working dilution should be determined for each specific assay condition.
• WB: 5-10 μg/ml
• IHC: 5-10 μg/ml
Tissue: Formalin-fixed Paraffin-embedded tissues
Pre-treatment: Citrate
Primary incubation: 24 hours at 4°C - Application Notes
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This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC).
*Expected Molecular Weight = 60 kD
WB positive control: Rat and mouse brain membrane lysates. This clone does not cross-react with human RGS14 in WB.
IHC positive control: Rat and mouse brain tissue. - Product Citations
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- RRID
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AB_2564854 (BioLegend Cat. No. 821801)
Antigen Details
- Structure
- Expected MW: 60kD
- Biology Area
- Cell Biology, Neuroscience, Synaptic Biology
- Molecular Family
- Postsynaptic proteins
- Gene ID
- 114705 View all products for this Gene ID
- UniProt
- View information about RGS14 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All RGS14 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-RGS14 | N133/21 | IHC-P,WB |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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