- Clone
- W20284A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- NR2B1, RXR alpha, RXR-alpha, Retinoid X receptor alpha, Nuclear receptor subfamily
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A_W20284A_PURE_RXRalpha_Antibody_WB_081622 A_W20284A_PURE_RXRalpha_Antibody_WB_081622](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W20284A_PURE_RXRalpha_Antibody_WB_081622.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Whole cell extracts (15 µg total protein per lane) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a nitrocellulose membrane, and probed with 1 µg/mL of purified anti-RXRα (clone W20284A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:25000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection reagent. Lane M: Molecular weight marker -
ICC staining of purified anti-RXRα (clone W20284A) on HaCaT cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.5% Triton X-100, and blocked with 5% FBS for 1 hour at room temperature. Cells were then intracellularly stained with 5.0 μg/mL of the primary antibody, followed by incubation with 2.5 μg/mL of Alexa Fluor® 488 goat anti-rat IgG (Cat. No. 405418) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801), and the image was captured with a 40X objective, Scale bar = 25μm.
Cat # | Size | Price | Save |
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606951 | 25 µg | ¥24,200 | |
606952 | 100 µg | ¥60,500 |
The human RXR (retinoid X receptor) includes three distinct genes (RXRα, β, and γ). RXR is composed of two major domains of nuclear receptor family proteins, the DNA-binding domain (DBD) and the ligand binding domain (LBD) that bind with high affinity to 9-cis –RA (Retinoic Acid). The RAR/RXR heterodimers bind to RA response elements (RARE) composed of two 5'-AGGTCA-3' sites to modulate RA-mediated gene expression.
The RXRα, also known as NR2B1, plays a crucial role in various biological effects, such as UVR-induced DNA damage, cell growth, development, metabolism, differentiation, and apoptosis. RXRα is highly expressed in liver and also expressed in kidney and brain. It regulates many intracellular receptor signaling pathways via interacting with ligands and co-regulators. The RXRα/PPARα heterodimer is essential for energy balance regulation such as PPARα transcriptional activity on fatty acid oxidation genes including ACOX1 and the P450 system genes. Proteolytic cleavage of RXRα (tRXRα) protein results in a truncated product found in many tumor cells. The tRXRα (RXRα-Δ80) interacts with the p85α subunit of the PI3K/AKT pathway and leads to the increase of tumor cell proliferation.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Partial Recombinant human RXRα protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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The clone does not cross-react with mouse and rat RXRα.
For ICC, we recommend 4% PFA fixation followed by permeabilization with 0.5% Triton X-100 or ice-cold methanol. We do not recommend ice-cold methanol for fixation and permeabilization.
The clone is not suitable for IHC. - RRID
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AB_2924620 (BioLegend Cat. No. 606951)
AB_2924620 (BioLegend Cat. No. 606952)
Antigen Details
- Structure
- RXRα is a 462 amino acid protein with a predicted molecular weight of 51 kD
- Distribution
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Expressed in lung fibroblasts, monocytes, kidney and brain
- Function
- Transcription factor, cell growth and apoptosis
- Interaction
- RARA, NCOA3, NCOA6, PELP1, SENP6, SFPQ, DNTTIP2, RNF8, BHLHE40/DEC1, BHLHE41/DEC2, NCOR1 and NCOR2
- Ligand/Receptor
- 9-cis retinoic acid
- Cell Type
- Fibroblasts, Monocytes
- Biology Area
- Transcription Factors
- Molecular Family
- Steroid Receptors/Nuclear Receptors
- Antigen References
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- Dawson MI, et al. 2012. Biochim Biophys Acta. 1821:21-56.
- Rastinejad F, et al. 2000. EMBO J. 19:1045-54.
- Romert A, et al. 1998. Proc Natl Acad Sci U S A. 95:4404–9.
- Gorla-Bajszczak A, et al. 1999. Mol Cell Endocrinol. 147:37-47.
- Wang Z, et al. 2011. J Invest Dermatol. 131:177-87.
- Zhang R, et al. 2018. Cell Death Dis. 9:532.
- Wendling O, et al. 1999. Proc Natl Acad Sci U S A. 96:547-51.
- Chen L, et al. 2018. Cell Mol Biol Lett. 23:36.
- Gene ID
- 6256 View all products for this Gene ID
- UniProt
- View information about RXRalpha on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All RXRα Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-RXRα | W20284A | WB,ICC |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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