- Clone
- 4F3-A8-D9 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Transducin Beta Like 1 X-Linked Receptor 1, F-Box-Like/WD Repeat-Containing Protein TBL1XR1, Transducin Beta-Like 1X-Related Protein 1, MRD41, DC42
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![4F3-A8-D9_PURE_TBLR1_Antibody_1_120219 4F3-A8-D9_PURE_TBLR1_Antibody_1_120219](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/4F3-A8-D9_PURE_TBLR1_Antibody_1_120219.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western blot of purified anti-TBLR1 antibody (clone 4F3-A8-D9). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain nuclear lysate; Lane 3: 20 µg of rat brain nuclear lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-TBLR1 antibody (clone 4F3-A8-D9) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 10.0 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-TBLR1 antibody (clone 4F3-A8-D9) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 10.0 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # | Size | Price | Save |
---|---|---|---|
870001 | 25 µg | ¥23,320 | |
870002 | 100 µg | ¥60,720 |
TBLR1 is a member of the WD40 repeat-containing family of proteins. It shares similarity with TBL1 in sequence, and is believed to be a component of the complex of nuclear corepressor and HDAC3. TBLR1 is involved in signaling pathway during development through the regulation of chromatin acetylation. It is also associated with diseases including pierpont syndrome, mental retardation, and autism spectrum disorders.
Product DetailsProduct Details
- Verified Reactivity
- Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Purified recombinant human TBLR1 protein fragments expressed in E. coli
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.2 - 0.5 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
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AB_2820184 (BioLegend Cat. No. 870001)
AB_2820185 (BioLegend Cat. No. 870002)
Antigen Details
- Structure
- TBLR1 is a 514 amino acid protein with a predicted and observed molecular mass of ~56 kD.
- Distribution
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Tissue Distribution: Brain, immune system, heart, and lung
Cellular Distribution: Nucleus - Function
- Chromatin acetylation
- Interaction
- HDAC3, TBL1, NCOR1, NCOR2, MECP2
- Cell Type
- B cells, Monocytes, Neurons, NK cells
- Biology Area
- Cancer Biomarkers, Cell Biology, Neurodegeneration, Neuroscience, Signal Transduction
- Molecular Family
- Nuclear Markers
- Antigen References
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- Xi X, et al. 2019. Eur J Pharmacol. 844:259-267.
- Nishi A, et al. 2017. Sci Rep. 7(1):2887.
- Gene ID
- 81004 View all products for this Gene ID 365755 View all products for this Gene ID
- UniProt
- View information about TBLR1 on UniProt.org
Other Formats
View All TBLR1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-TBLR1 | 4F3-A8-D9 | WB,IHC-P |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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