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![Western_ready_ECL_Substrate_Premium_kit_1_063020 Western_ready_ECL_Substrate_Premium_kit_1_063020](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/Western_ready_ECL_Substrate_Premium_kit_1_063020.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western blot analysis using Western-Ready™ ECL Substrate Plus and Premium Kits: The indicated amounts of whole cell extracts from HeLa cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.1 µg/mL (1:5000 dilution) of purified anti-Lamin A/C antibody (clone WL4G10) (Cat. No. 600002) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution, followed by incubation with Western-Ready™ ECL Substrate Plus Kit or Western-Ready™ ECL Substrate Premium Kit. -
ELISA analysis using Western-Ready™ ECL Substrate Plus and Premium Kits: The indicated amounts of purified anti-SSRP1 antibody (clone 10D1) (Cat. No. 609702) were captured by anti-mouse FC -coated 96-well plates for 1 hour at room temperature. Bound antibody was detected with HRP goat anti-mouse IgG antibody (Cat. No. 405306) using either Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) (blue triangles) or Western-Ready™ ECL Substrate Plus Kit (red circles). Shown values represent the average signal of three technical replicates after background subtraction. -
ELISA analysis using Western-Ready™ ECL Substrate Plus and Premium Kits: Bar graph representation of low picogram to high femtogram values shown in panel C.
Cat # | Size | Price | Save |
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426318 | 20 mL | ¥19,580 | |
426319 | 200 mL | ¥128,920 |
Western-Ready™ ECL Substrate Premium Kit is a sensitive non-radioactive, enhanced luminol-based chemiluminescent substrate (ECL) for the detection of horseradish peroxidase (HRP) in immunoblotting and other immunoassay applications. The ECL substrate detects femtogram amounts of antigen with stable light output (up to 4 hours), which can be visualized using X-ray films or CCD-based imagers.
Product DetailsProduct Details
- Formulation
- Proprietary
- Preparation
- To prepare working solution, mix equal volumes of Western-Ready™ ECL Substrate Premium Solution A with Western-Ready™ ECL Substrate Premium Solution. Add 0.1 mL of working solution per cm2 of membrane, and uniformly distribute over the entire membrane surface.
- Storage & Handling
- Ambient room temperature
- Application
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WB - Quality tested
ELISA - Verified - Application Notes
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BioLegend offers two luminol-based enhanced chemiluminescent substrate solution packages for the detection of horseradish peroxidase on immunoblots and ELISA plates: Western-Ready™ ECL Substrate Plus Kit and Western-Ready™ ECL Substrate Premium Kit. The Western-Ready™ ECL Substrate Plus Kit detects mid- to –low picogram levels of antigen, while the Western-Ready™ ECL Substrate Premium Kit detects high femtogram levels of antigen and is suitable for applications where more sensitive detection is required.
- Additional Product Notes
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20 mL size (Cat. No. 426318) contains 10 mL each of solution A and solution B
200 mL size (Cat. No. 426319) contains 100 mL each of solution A and solution B - Product Citations
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Antigen Details
- Gene ID
- NA
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