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Alexa Fluor® 488 anti-mouse/human CD45R/B220 Antibody

Pricing & Availability
Clone
RA3-6B2 (See other available formats)
Regulatory Status
RUO
Other Names
B220
Isotype
Rat IgG2a, κ
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Product Citations
publications
1_RA3-6B2_AF488_090507
C57BL/6 mouse splenocytes stained with RA3-6B2 Alexa Fluor® 488
  • 1_RA3-6B2_AF488_090507
    C57BL/6 mouse splenocytes stained with RA3-6B2 Alexa Fluor® 488
  • 2_RA3-6B2_A488_B220_Antibody_IHC-F_080519
    C57BL/6 mouse frozen lymph node section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS plus 5% rat serum for 1 hour at room temperature. Then the section was stained with 5 µg/ml of CD8 (clone 53-6.7) Alexa Fluor® 594 (red), 5 µg/ml of CD4 (clone GK1.5) Alexa Fluor® 647 (blue), and 5 µg/ml of B220 (clone RA3-6B2) Alexa Fluor® 488 (green) overnight at 4°C. The image was captured by 10X objective.
  • 3_RA3-6B2_A488_CD45R-B220_Antibody_3_092021.png
    Paraformaldehyde-fixed (4%), 500 µm-thick mouse spleen section was processed according to the Ce3D™ Tissue Clearing Kit protocol (Cat. No. 427701). The section was costained with anti-mouse/human CD45R/B220 Antibody (clone RA3-6B2) Alexa Fluor® 488 at 5 µg/mL (green), anti-mouse CD68 Antibody (clone FA-11) Alexa Fluor® 594 at 5 µg/mL (blue), and anti-mouse CD3ε Antibody (clone 145-2C11) Alexa Fluor® 647 at 5 µg/mL (magenta). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
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See Alexa Fluor® 488 spectral data
Cat # Size Price Save
103228 25 µg ¥17,980
103225 100 µg ¥41,300
Description

CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse,Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Abelson murine leukemia virus-induced pre-B tumor cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F, 3D IHC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 5.0 μg/ml is suggested. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo modulation of B cell responses2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6, and spatial biology (IBEX)14,15.

Application References

(PubMed link indicates BioLegend citation)
  1. Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
  2. George A, et al. 1994. J. Immunol. 152:1014. (Activ)
  3. Asensi V, et al. 1989. Immunology 68:204. (Activ)
  4. Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Chang C L-T, et al. 2007. J. Immunol. 178:6984.
  9. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  10. Lang GL, et al. 2008. Blood 111:2158. PubMed
  11. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  12. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  13. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  14. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  15. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Mooney C, et al. 2017. International Journal of Molecular Sciences. 10.3390/ijms18051037. PubMed
  2. Shimoda A, et al. 2017. Biochem Biophys Res Commun. 10.1016/j.bbrc.2017.07.126. PubMed
  3. Wang J, et al. 2018. Front Immunol. 9:371. PubMed
  4. Thurlow LR, et al. 2018. Cell Host Microbe. 24:261. PubMed
  5. Hu J, et al. 2019. Mol Ther Nucleic Acids. 16:650. PubMed
  6. Ooi JD, et al. 2019. Nat Commun. 10:3392. PubMed
  7. Chen F et al. 2018. Cell reports. 25(10):2775-2783 . PubMed
  8. Uribesalgo I, et al. 2019. EMBO Mol Med. 11:e9266. PubMed
  9. Nunes NS, et al. 2019. EBioMedicine. 45:495. PubMed
  10. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  11. Tsai F, et al. 2017. J Exp Med. 214:3753. PubMed
  12. Tanaka Y, et al. 2017. J Immunol. 199:4016. PubMed
  13. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  14. Chen C, et al. 2019. Cell Rep. 29:4200. PubMed
  15. Si Y, et al. 2018. J Control Release. 282:120. PubMed
  16. Pham THM, et al. 2020. Cell Host & Microbe. 27(1):54-67.e5.. PubMed
  17. Lian J, et al. 2020. Cell Reports. 31(8):107679. PubMed
  18. Gordan S, et al. 2020. Cell Reports. 29(10):3033-3046.e4.. PubMed
  19. Ge S, et al. 2012. Nephrol Dial Transplant. 209:35:00. PubMed
  20. Luo Z, et al. 2013. J Control Release. 170:259. PubMed
  21. Wang C, et al. 2014. Vaccine. 32:5475. PubMed
  22. Lin J, Sorger M 2015. Sci Rep. 6: 8390. PubMed
  23. Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed
  24. INAGAWA H, et al. 2016. In Vivo. 30: 205 - 211. PubMed
  25. AR P, et al. 2016. Circ Res. 118: 400-409. PubMed
  26. Frasch S, et al. 2016. J Immunol. 197: 1425 - 1434. PubMed
  27. Cosgrove J, et al. 2020. Nat Commun. 3.011805556. PubMed
  28. Girón-Pérez DA, et al. 2020. J Cell Sci. 133:00:00. PubMed
  29. Luo L, et al. 2021. J Neuroinflammation. 18:27. PubMed
  30. Tummers B, et al. 2020. Immunity. 52(6):994-1006.e8. PubMed
  31. Kim SH, et al. 2021. Cell Reports. 35(2):108995. PubMed
  32. Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed
  33. Werner A, et al. 2021. iScience. 24:103076. PubMed
  34. Turner JA, et al. 2020. Immunity. 53:1202. PubMed
  35. Schnoor M, et al. 2020. Bio Protoc. 10:e3842. PubMed
  36. Mostofa A, et al. 2021. JCI Insight. 6:. PubMed
  37. Hu M, et al. 2020. Cancer Immunol Res. 8:1150. PubMed
  38. Ceglia V, et al. 2021. J Immunol. 207:2060. PubMed
  39. Li N, et al. 2021. iScience. 24:103163. PubMed
  40. Chen B, et al. 2021. Cell. 184:6262. PubMed
  41. Trindade BC, et al. 2021. Immunity. 54:2273. PubMed
  42. Serger E, et al. 2022. Nature. 607:585. PubMed
  43. Kitai H, et al. 2022. BMC Biol. 20:248. PubMed
  44. Shibata K, et al. 2022. Nat Commun. 13:6948. PubMed
  45. Du Y, et al. 2022. J Exp Med. 219: . PubMed
  46. Hao H, et al. 2023. Nat Biomed Eng. . PubMed
  47. Kang YA, et al. 2023. J Exp Med. 220:. PubMed
RRID
AB_492874 (BioLegend Cat. No. 103228)
AB_389308 (BioLegend Cat. No. 103225)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

B cells, T cell subset, NK cell subset

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID 5788 View all products for this Gene ID
UniProt
View information about CD45R on UniProt.org
Go To Top Version: 6    Revision Date: 09/20/2021

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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