- Clone
- RMG2a-62 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Immunoglobulin G2a
- Isotype
- Rat IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![RMG2a-62_A488_IgG2a_Antibody_101917 RMG2a-62_A488_IgG2a_Antibody_101917](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/RMG2a-62_A488_IgG2a_Antibody_101917.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were stained with purified anti-human CD3 (clone HIT3a isotype mouse IgG2a, κ) followed by Alexa Fluor® 488 anti-mouse IgG2a (filled histogram) or Alexa Fluor® 488 anti-mouse IgG2a Antibody alone (open histogram).
Cat # | Size | Price | Save |
---|---|---|---|
407121 | 25 µg | ¥19,580 | |
407122 | 100 µg | ¥42,680 |
The RMG2a-62 monoclonal antibody reacts with immunoglobulin G2a and G2c (IgG2a, IgG2c) and in all tested mouse haplotype (Igh-a and b). It does not react with other isotypes. The RMG2a-62 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.
Product DetailsProduct Details
- Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse Ig cocktail
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
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Blue Laser (488 nm)
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Application References
(PubMed link indicates BioLegend citation) -
- Gavin AL, et al.2006. Science 314:1936.
- Podolin PL, et al. 2008. J. Immunol. 180:7989. (IP) PubMed
- Product Citations
- RRID
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AB_2721367 (BioLegend Cat. No. 407121)
AB_2721368 (BioLegend Cat. No. 407122)
Related FAQs
- IgG2a gene is deleted in some mouse strains, which ones are they?
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IgG2a gene is deleted in C57Bl/6, C57Bl/10, SJL, and NOD mice. It is replaced with IgG2c gene instead.
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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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