- Clone
- Poly4064 (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Donkey Polyclonal Ig
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![Poly4064_AF488_IgG_ANtibody_FC_022014.jpg Poly4064_AF488_IgG_ANtibody_FC_022014.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/Poly4064_AF488_IgG_ANtibody_FC_022014.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 splenocytes were stained with NK-1.1 Brilliant Violet 421™ and purified rabbit anti-Asialo-GM1, followed by donkey anti-rabbit IgG (clone Poly4064) Alexa Fluor® 488 (top image).
Cat # | Size | Price | Save |
---|---|---|---|
406416 | 100 µg | ¥31,020 |
This polyclonal donkey anti-rabbit IgG antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other subclasses of rabbit immunoglobulins.
Product DetailsProduct Details
- Reactivity
- Rabbit
- Antibody Type
- Polyclonal
- Host Species
- Donkey
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
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Blue Laser (488 nm)
- Application Notes
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This conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.
- Product Citations
- RRID
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AB_2563203 (BioLegend Cat. No. 406416)
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