- Clone
- Poly4064 (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Donkey Polyclonal Ig
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![Poly4064_AF555_IgG_Antibody_IF_030515 Poly4064_AF555_IgG_Antibody_IF_030515](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/Poly4064_AF555_IgG_Antibody_IF_030515.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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HeLa cells were fixed with 1% paraformaldehyde for ten minutes followed by permeabilization for ten minutes with 0.5% Triton X-100, and then blocked with 5% FBS for 30 minutes. 2.5 µg/ml of purified BRCA-1 (Poly26599) was added to the cells and left at 4°C overnight. The cells were then stained with 2.5 µg/ml of Alexa Fluor® 555 (Yellow) for 2 hours at room temperature. Nuclei were counterstained with DAPI and are shown in blue and phalloidin shown in red. The image was captured with a 40X objective.
Cat # | Size | Price | Save |
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406412 | 100 µg | ¥31,020 |
This polyclonal donkey anti-rabbit IgG antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other subclasses of rabbit immunoglobulins.
Product DetailsProduct Details
- Reactivity
- Rabbit
- Antibody Type
- Polyclonal
- Host Species
- Donkey
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 555 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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ICC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 555 has an excitation maximum of 555 nm, and a maximum emission of 565 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
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Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
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This conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.
- Product Citations
- RRID
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AB_2563181 (BioLegend Cat. No. 406412)
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