- Clone
- C1.7 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- VII 70350
- Other Names
- 2B4, SLAMF4
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![C1dot7_A700_CD244_Antibody_1_062317 C1dot7_A700_CD244_Antibody_1_062317](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/C1dot7_A700_CD244_Antibody_1_062617.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were stained with CD8 FITC and CD244 (2B4) (clone C1.7) Alexa Fluor® 700 (top) or mouse IgG1, κ Alexa Fluor® 700 isotype control (bottom). -
Cat # | Size | Price | Save |
---|---|---|---|
329525 | 25 tests | ¥32,340 | |
329526 | 100 tests | ¥76,340 |
CD244, known as 2B4, is a 38 kD type I transmembrane protein. It is a member of the CD2 subset of the immunoglobulin superfamily (IgSF) molecules. CD244 is expressed on NK cells, a subset of T cells (including most CD8+ T cells and γ/δ T cells), monocytes, basophils, and eosinophils. CD48 is the ligand of CD244. It has been reported that ligation of human CD244 results in enhanced NK cell cytotoxicity and cytokine production. Recent studies have shown that human CD244, like murine CD244, has both activating and inhibitory functions, which are dependent on the density of surface 2B4 expression, degree of ligation, and the level of the adaptor molecule SAP expression.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
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Red Laser (633 nm)
- Application Notes
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Additional reported applications (for the relevant formats) include: inducing cytokine production by NK cells, enhancing NK cytotoxicity, and Western blotting.
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Application References
(PubMed link indicates BioLegend citation) - Product Citations
- RRID
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AB_2687331 (BioLegend Cat. No. 329525)
AB_2687332 (BioLegend Cat. No. 329526)
Antigen Details
- Structure
- 38 kD type I transmembrane protein, CD2 family member, Ig superfamily
- Distribution
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NK, T subset, monocytes, basophils, and eosinophils
- Function
- Activation and inhibition of NK cell function
- Ligand/Receptor
- CD48
- Cell Type
- Basophils, Eosinophils, Monocytes, NK cells, T cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
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1. Lee KM, et al. 2003. J. Immunol. 170:4881.
2. Chlewicki LK, et al. 2008. J. Immunol. 180:8159.
3. Messmer B, et al. 2006. J. Immunol. 176:4646.
4. Boles KS, et al. 2001. Immunol. Rev. 181:234. - Gene ID
- 51744 View all products for this Gene ID
- UniProt
- View information about CD244 on UniProt.org
Related Pages & Pathways
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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