- Clone
- HIP1 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- IV P70
- Other Names
- Glycocalicin, gpIbα
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![HIP1_A700_CD42b_Antibody_080717 HIP1_A700_CD42b_Antibody_080717](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/HIP1_A700_CD42b_Antibody_080717.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human platelets were stained with CD42b (clone HIP1) Alexa Fluor® 700 (filled histogram) or mouse IgG1, κ Alexa Fluor® 700 isotype control (open histogram).
Cat # | Size | Price | Save |
---|---|---|---|
303927 | 25 tests | ¥31,020 | |
303928 | 100 tests | ¥78,760 |
CD42b is a 145 kD glycoprotein known as gpIbα. It is covalently bonded to CD42c to form GPIb. CD42b is expressed on platelets and megakaryocytes. CD42b/c heterodimer forms a complex with CD42a and d and acts as the receptor for von Willibrand factor and thrombin.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
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Clone HIP1 recognizes an epitope within the N-terminal region of the GPIba chain5. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, Westerm blotting, and inhibition of platelet aggregation2. The Ultra LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 303939).
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Application References
(PubMed link indicates BioLegend citation) - Product Citations
- RRID
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AB_2715928 (BioLegend Cat. No. 303927)
AB_2715929 (BioLegend Cat. No. 303928)
Antigen Details
- Structure
- Mucin, leucine-rich repeat family, 145 kD
- Distribution
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Platelets, megakaryocytes
- Function
- Complex with CD42a, c and d, platelet adhesion and activation
- Ligand/Receptor
- Von Willebrand factor, thrombin
- Cell Type
- Megakaryocytes, Platelets
- Biology Area
- Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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1. Clemetson K, et al. 1982. J. Clin. Invest. 70:304.
2. Fox J, et al. 1988. J. Biol. Chem. 263:4882.
3. Kuijpers R, et al. 1992 Blood 79:283. - Gene ID
- 2812 View all products for this Gene ID
- UniProt
- View information about CD42b on UniProt.org
Related Pages & Pathways
Pages
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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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