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Alexa Fluor® 700 anti-mouse/human CD45R/B220 Antibody

Pricing & Availability
Clone
RA3-6B2 (See other available formats)
Regulatory Status
RUO
Other Names
B220
Isotype
Rat IgG2a, κ
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Product Citations
publications
RA3-6B2_Alx700_080408.jpg
C57BL/6 mouse splenocytes stained with RA3-6B2 Alexa Fluor® 700
  • RA3-6B2_Alx700_080408.jpg
    C57BL/6 mouse splenocytes stained with RA3-6B2 Alexa Fluor® 700
See Alexa Fluor® 700 spectral data
Cat # Size Price Save
103231 25 µg ¥16,740
103232 100 µg ¥37,560
Description

CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse,Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Abelson murine leukemia virus-induced pre-B tumor cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The CD45R antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633nm / 635nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo modulation of B cell responses2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6, and spatial biology (IBEX)14,15.

Application References

(PubMed link indicates BioLegend citation)
  1. Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
  2. George A, et al. 1994. J. Immunol. 152:1014. (Activ)
  3. Asensi V, et al. 1989. Immunology 68:204. (Activ)
  4. Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Chang C L-T, et al. 2007. J. Immunol. 178:6984.
  9. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  10. Lang GL, et al. 2008. Blood 111:2158. PubMed
  11. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  12. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  13. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  14. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  15. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Rydyznski CE et al. 2018. Cell reports. 24(13):3367-3373 . PubMed
  2. Perry JSA, et al. 2018. Immunity. 48:923. PubMed
  3. Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
  4. Adachi Y, et al. 2019. Nat Commun. 10:3883. PubMed
  5. Onodera T, et al. 2019. J Immunol. 203:3282. PubMed
  6. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  7. Fallet B, et al. 2020. Cell Rep. 30:1013. PubMed
  8. Chauveau A, et al. 2020. Immunity. 52:794. PubMed
  9. Moufarrij S, et al. 2020. Sci Rep. 10:3470. PubMed
  10. Ghezraoui H, et al. 2018. Nature. 560:122. PubMed
  11. Hong JP, et al. 2020. Cell Reports Medicine. 1(3):100035. PubMed
  12. Boyoglu-Barnum S, et al. 2014. J Virol. 88:10569. PubMed
  13. Adachi Y, et al. 2015. J Exp Med. 212: 1709-1723. PubMed
  14. Onodera T, et al. 2016. J Immunol. 196: 4172 - 4184. PubMed
  15. Shang Y, et al. 2016. Nat Immunol. 10.1038/ni.3486. PubMed
  16. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
  17. Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed
  18. Kumari P, et al. 2021. Cell Reports. 35(3):109012. PubMed
  19. Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
  20. Szodoray P, et al. 2021. Cell Reports. 36(6):109525. PubMed
  21. Wiesner DL, et al. 2020. Cell Host Microbe. 614:27. PubMed
  22. La Rose AM, et al. 2021. Mol Metab. 53:101265. PubMed
  23. Panea C, et al. 2021. Commun Biol. 4:913. PubMed
  24. Tsyklauri O, et al. 2021. EMBO Rep. 22:e50785. PubMed
  25. Yang Y, et al. 2021. Nat Commun. 12:525. PubMed
  26. Yamin R, et al. 2021. Nature. 599:465. PubMed
  27. Agrawal M, et al. 2021. Cells. 10:. PubMed
  28. Wang X, et al. 2021. Sci Transl Med. 13:. PubMed
  29. Groenen AG, et al. 2022. J Lipid Res. 63:100167. PubMed
  30. Spix B, et al. 2022. Nat Commun. 13:318. PubMed
  31. Liu Y, et al. 2022. Nat Commun. 13:2665. PubMed
  32. Lee S, et al. 2021. Autophagy. 18:1062. PubMed
  33. Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
  34. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  35. Ruf B, et al. 2021. Cancer Immunol Res. 9:1024. PubMed
  36. Liu Q, et al. 2021. Adv Mater. 33:e2102852. PubMed
  37. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  38. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  39. Knizkova D, et al. 2022. Nat Immunol. 23:1644. PubMed
  40. Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed
  41. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
  42. Peauroi EM, et al. 2022. iScience. 25:105510. PubMed
  43. MacLean AJ, et al. 2022. Immunity. 55:718. PubMed
  44. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  45. Hao J, et al. 2022. Cell Rep. 41:111804. PubMed
  46. Feng J, et al. 2022. Immunity. 55:405. PubMed
  47. Schönberger K, et al. 2023. Anal Chem. 95:4325. PubMed
RRID
AB_493716 (BioLegend Cat. No. 103231)
AB_493717 (BioLegend Cat. No. 103232)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

B cells, T cell subset, NK cell subset

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID 5788 View all products for this Gene ID
UniProt
View information about CD45R on UniProt.org
Go To Top Version: 3    Revision Date: 06/27/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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