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APC anti-human CD197 (CCR7) Antibody

Pricing & Availability
Clone
G043H7 (See other available formats)
Regulatory Status
RUO
Other Names
BLR2, CDw197, EBI1, CMKBR7
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
G043H7_APC_1_112811
Human peripheral blood lymphocytes were stained with CD3 FITC and CCR7 (clone G043H7) APC (top) or mouse IgG2a, κ APC isotype control (bottom).
  • G043H7_APC_1_112811
    Human peripheral blood lymphocytes were stained with CD3 FITC and CCR7 (clone G043H7) APC (top) or mouse IgG2a, κ APC isotype control (bottom).
  • G043H7_APC_2_112811
  • G043H7_PE_CCR7_Antibody_050222
    APC anti-human CD197 (CCR7) Antibody Cynomolgus monkey peripheral blood lymphocytes were stained with anti‐human CCR7 APC (clone G043H7) (filled histogram) or mouse IgG2a, κ isotype control (open histogram).
  • G043H7_PE_CCR7_Antibody_3_050222
    Rhesus macaque peripheral blood lymphocytes were stained with anti‐human CCR7 APC (clone G043H7) (filled histogram) or mouse IgG2a, κ isotype control (open histogram).
See APC spectral data
Cat # Size Price Save
353213 25 tests ¥30,140
353214 100 tests ¥74,360
Description

CCR7, also known as CD197, is a chemokine receptor that binds CCL19 and CCL21. CCR7 and its ligands link innate and adaptive immunity by affecting interactions between T cells and dendritic cells and their downstream effect. Naïve T cells enter the lymph node through high endothelial venules, which express CCL21. Dendritic cells and macrophages enter the lymph node through afferent lymphatics. The encounter of T cells and dendritic cells in the T cell zone is CCR7-dependent. In addition, during immunological surveillance, B cells recirculate between B-cell-rich compartments (follicles or B cell zones) in secondary lymphoid organs, surveying for antigen. After antigen binding, B cells move to the boundary of B and T zones to interact with T-helper cells; this B cell migration is directed by CCR7 and its ligands. CCR7-positive cancer cell expression has been associated with lymph node metastasis.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
CCR7-transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Product Citations
  1. Bradley T et al. 2018. Cell reports. 25(1):107-117 . PubMed
  2. Fajgenbaum DC, et al. 2019. J Clin Invest. 130:4451. PubMed
  3. Chiu YL, et al. 2018. Immun Ageing. 15:27. PubMed
  4. Blengio F, et al. 2013. Immunobiology. 218:76. PubMed
  5. Soriano-Sarabia N, et al. 2014. J Virol. 88:14070. PubMed
  6. Yin S, et al. 2015. Sci Rep. 5: 14432. PubMed
  7. Koh WH, et al. 2020. STAR Protoc. 1:100203. PubMed
  8. Dai X, et al. 2021. Bioact Mater. 1.659027778. PubMed
  9. Luo Y, et al. 2021. Nat Commun. 12:3913. PubMed
  10. Cho JH, et al. 2021. Nat Commun. 12:792. PubMed
  11. Mao FY, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:395. PubMed
  12. Liu C, et al. 2021. Cell Res. 31:1106. PubMed
  13. Li M, et al. 2021. J Clin Invest. 131:. PubMed
  14. Miao L, et al. 2021. Clin Transl Med. 11:e395. PubMed
  15. Muliaditan T, et al. 2021. Cell Rep Med. 2:100457. PubMed
  16. Guo J, et al. 2022. Front Cell Dev Biol. 9:775599. PubMed
  17. Shen Y, et al. 2021. Cell Stress Chaperones. 26:937. PubMed
  18. Mysore V, et al. 2021. Med (N Y). 2:1050. PubMed
  19. Andonian BJ, et al. 2022. Sci Rep. 12:7450. PubMed
  20. Jo T, et al. 2022. Cancer Immunol Immunother. Online ahead of print. PubMed
  21. Ronaghan NJ, et al. 2022. PLoS One. 17:e0276013. PubMed
  22. Gartshteyn Y, et al. 2023. Life Sci Alliance. 6: . PubMed
  23. Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed
  24. Prockop SE, et al. 2023. J Clin Invest. 133:. PubMed
RRID
AB_10915474 (BioLegend Cat. No. 353213)
AB_10917387 (BioLegend Cat. No. 353214)

Antigen Details

Structure
Chemokine receptor, G protein-coupled receptors (GPCR), seven transmembrane receptor.
Distribution

T cells, B cells, NK, dendritic cells.

Function
The chemokine receptor CCR7 plays a pivotal role in the homing of naïve T cells and regulatory T cells to secondary lymphoid organs, and the migration of dendritic cells into afferent lymphatic vessels.
Ligand/Receptor
CCL19 and CCL21.
Cell Type
B cells, Dendritic cells, NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Yanagihara S, et al. 1998. J. Immunol. 161:3096.
2. Charo IF, et al. 2006. N. Engl. J. Med. 354:610.
3. Reif K, et al. 2002. Nature 416:94.
4. Nakata B, et al. 2008. Oncology 74:69.
5. Brodie T. et al. 2013. Cytometry A. 6: 530-2. PubMed
6. Graves A.J. et al. 2014. Cytometry A. 7: 576–9 PubMed
7. Moncunill G. et al. 2014. Cytometry A. 12: 995-8 PubMed

Gene ID
1236 View all products for this Gene ID
UniProt
View information about CD197 on UniProt.org

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Go To Top Version: 2    Revision Date: 04/08/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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