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APC anti-human HLA-E Antibody

Pricing & Availability
Clone
3D12 (See other available formats)
Regulatory Status
RUO
Other Names
HLA class I histocompatibility antigen, alpha chain E, HLAE
Isotype
Mouse IgG1, κ
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Product Citations
publications
3D12_APC_HLA-E_Antibody_FC_122214
Human peripheral blood lymphocytes were stained with HLA-E (clone 3D12) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
  • 3D12_APC_HLA-E_Antibody_FC_122214
    Human peripheral blood lymphocytes were stained with HLA-E (clone 3D12) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
See APC spectral data
Cat # Size Price Save
342605 25 tests ¥24,860
342606 100 tests ¥64,020
Description

HLA-E is a non-classical MHC class I (MHC-Ib) molecule. It is characterized by limited polymorphism and ubiquitous expression. HLA-E, as other MHC-I molecules, is heterodimerized with β2-microglobulin. HLA-E interacts with CD94/NKG2 receptor to regulate NK cell cytolysis activities.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Lee N, et al. 1998. Proc. Natl. Acad. Sci. USA. 95:5199.
  2. Wooden SL, et al. 2005. J. Immunol. 175:1383.
  3. Monaco EL, et al. 2008. J. Immunol. 181:5442.
  4. Corrah TW, et al. 2011. J. Virol. 85:3367. PubMed
Product Citations
  1. Ng W, et al. 2021. Pharm Biol. 59:47:00. PubMed
  2. Li YR, et al. 2021. Cell Rep Med. 2:100449. PubMed
  3. Wu D, et al. 2020. Biomark Res. 8:3. PubMed
  4. Yamaguchi S, et al. 2021. Eur J Immunol. 51:2306. PubMed
  5. Hu X, et al. 2023. Nat Biotechnol. . PubMed
RRID
AB_2565260 (BioLegend Cat. No. 342605)
AB_2565261 (BioLegend Cat. No. 342606)

Antigen Details

Structure
HLA family, Ig superfamily, 42 kD
Distribution

Broadly expressed

Function
Mediate cell recognition by NK cells
Ligand/Receptor
CD94/NKG2
Biology Area
Immunology
Molecular Family
MHC Antigens
Antigen References

1. Sullivan LC, et al. 2008. Tissue Antigen. 72:415.
2. Koller BH, et al. 1988. J. Immunol. 141:897.

Gene ID
3133 View all products for this Gene ID
UniProt
View information about HLA-E on UniProt.org
Go To Top Version: 1    Revision Date: 01/02/2015

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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