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APC/Fire™ 750 anti-mouse CD8a Antibody

Pricing & Availability
Clone
53-6.7 (See other available formats)
Regulatory Status
RUO
Other Names
T8, Lyt2, Ly-2
Isotype
Rat IgG2a, κ
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Product Citations
publications
53-6.7_APCFire750_CD8a_Antibody_1_032116
C57BL/6 thymocytes were stained with CD4 PE and CD8a (clone 53-6.7) APC/Fire™ 750 (top) or Rat IgG2a, κ APC/Fire™ 750 isotype control (bottom).
  • 53-6.7_APCFire750_CD8a_Antibody_1_032116
    C57BL/6 thymocytes were stained with CD4 PE and CD8a (clone 53-6.7) APC/Fire™ 750 (top) or Rat IgG2a, κ APC/Fire™ 750 isotype control (bottom).
  • 53-6.7_APCFire750_CD8a_Antibody_2_032116
See APC/Fire™ 750 spectral data
Cat # Size Price Save
100765 25 µg ¥21,560
100766 100 µg ¥47,520
Description

CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.

Application Notes

Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes3. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+ cells. Additional reported applications (for the relevant formats) include: immunoprecipitation1,3, in vivo and in vitro cell depletion2,10,15, inhibition of CD8 T cell proliferation3, blocking of cytotoxicity3,4, immunohistochemical staining5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)29,30. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).

Application References

(PubMed link indicates BioLegend citation)
  1. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IHC, IP)
  2. Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete)
  3. Takahashi K, et al. 1992. P. Natl. Acad. Sci. USA 89:5557. (Block, IP)
  4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503. (Block)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Kamimura D, et al. 2006. J. Immunol. 177:306.
  9. Bouwer HGA, et al. 2006. P. Natl. Acad. Sci. USA 103:5102. (FC, Deplete)
  10. Kao C, et al. 2005. Int. Immunol. 17:1607. PubMed
  11. Ko SY, et al. 2005. J. Immunol. 175:3309. (FC) PubMed
  12. Rasmussen JW, et al. 2006. Infect. Immun. 74:6590. PubMed
  13. Lee CH, et al. 2009. Clin. Cancer Res. PubMed
  14. Geiben-Lynn R, et al. 2008. Blood 112:4585. (Deplete) PubMed
  15. Kingeter LM, et al. 2008. J. Immunol. 181:6244. PubMed
  16. Guo Y, et al. 2008. Blood 112:480. PubMed
  17. Andrews DM, et al. 2008. J. Virol. 82:4931. PubMed
  18. Britschqui MR, et al. 2008. J. Immunol. 181:7681. PubMed
  19. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  20. Jordan JM, et al. 2008. Infect. Immun. 76:3717. PubMed
  21. Todd DJ, et al. 2009. J. Exp. Med. 206:2151. PubMed
  22. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  23. Medyouf H, et al. 2010. Blood 115:1175. PubMed
  24. Riedl P, et al. 2009. J. Immunol. 183:370. PubMed
  25. Apte SH, et al. 2010. J. Immunol. 185:998. PubMed
  26. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  27. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  28. Cui L, et al. 2015. J Control Release. 206:220. PubMed
  29. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  30. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Bengsch B et al. 2018. Immunity. 48(5):1029-1045 . PubMed
  2. Huang L, et al. 2018. Exp Neurol. 300:41. PubMed
  3. Abbott RK, et al. 2018. Immunity. 48:133. PubMed
  4. Yap JY, et al. 2018. Immunology. 154:522. PubMed
  5. Knox T, et al. 2019. Sci Rep. 9:6136. PubMed
  6. Ficht X, et al. 2019. J Immunol. 203:2377. PubMed
  7. Alter C et al. 2019. Am J Physiol Heart Circ Physiol. 317(1):H190-H200 . PubMed
  8. Gupta SS, et al. 2019. Cell Rep. 29:1862. PubMed
  9. Marro BS, et al. 2019. Cell Rep. 29:3293. PubMed
  10. Moufarrij S, et al. 2020. Sci Rep. 10:3470. PubMed
  11. Cibrián D, et al. 2020. J Allergy Clin Immunol. 145:199. PubMed
  12. Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed
  13. Lee AK, et al. 2020. Radiat Res. 193:305. PubMed
  14. Aryal B, et al. 2016. Nat Commun. 7:12313. PubMed
  15. Liu X, et al. 2020. Nature. . PubMed
  16. Wirasinha RC, et al. 2021. J Exp Med. 218: . PubMed
  17. Marinescu CI, et al. 2021. Stem Cell Res Ther. 12:319. PubMed
  18. Sugita Y, et al. 2021. Oncol Rep. 45:. PubMed
  19. Almutairi F, et al. 2021. Front Immunol. 12:772288. PubMed
  20. Pan D, et al. 2022. Cancer Res. 82:2748. PubMed
  21. Mittal A, et al. 2021. Sci Rep. 11:10731. PubMed
  22. Pfenninger P, et al. 2022. Front Immunol. 13:777113. PubMed
  23. Szeto C, et al. 2022. Nat Commun. 13:4951. PubMed
  24. Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed
  25. Toomer G, et al. 2022. Viruses. 14:. PubMed
  26. Lee JH, et al. 2022. Cell Rep. 38:110485. PubMed
  27. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  28. Gupta T, et al. 2023. Front Immunol. 14:1044703. PubMed
  29. Gao X, et al. 2023. Front Immunol. 14:1142428. PubMed
RRID
AB_2572112 (BioLegend Cat. No. 100765)
AB_2572113 (BioLegend Cat. No. 100766)

Antigen Details

Structure
Ig superfamily, CD8α chain, 34 kD
Distribution

Most thymocytes, T cell subset, some NK cells, lymphoid dendritic cells

Function
Co-receptor for TCR
Ligand/Receptor
MHC class I molecule
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Zamoyska R. 1994. Immunity 1:243.
3. Ellmeier W, et al. 1999. Annu. Rev. Immunol. 17:523.

Gene ID
12525 View all products for this Gene ID
UniProt
View information about CD8alpha on UniProt.org

Related FAQs

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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