Loading...

Biotin anti-mouse CD3ε Antibody

Pricing & Availability
Clone
145-2C11 (See other available formats)
Regulatory Status
RUO
Other Names
CD3ε, T3, CD3
Isotype
Armenian Hamster IgG
Ave. Rating
Submit a Review
Product Citations
publications
1_145-2C11_Biotin_021606
C57BL/6 mouse splenocytes were stained with biotinylated CD3e (clone 145-2C11) (filled histogram) or Armenian hamster IgG isotype control (open histogram), followed by Sav-PE.
  • 1_145-2C11_Biotin_021606
    C57BL/6 mouse splenocytes were stained with biotinylated CD3e (clone 145-2C11) (filled histogram) or Armenian hamster IgG isotype control (open histogram), followed by Sav-PE.
  • 2_145-2C11_Biotin_CD3e_Antibody_2_010620
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of biotin anti-mouse CD3ε (clone 145-2C11), Alexa Fluor® 594 anti-mouse B220 (clone RA3-6B2) (blue), and Alexa Fluor® 488 anti-mouse CD29 (clone HMβ1-1) (green) overnight at 4°C, followed by 2.5 µg/ml of Spark YG™ 570 Streptavidin (red) for 2 hours at room temperature. The image was captured by 10X objective.
Cat # Size Price Save
100303 50 µg ¥7,480
100304 500 µg ¥26,620
Description

CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl. For immunohistochemistry, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 145-2C11 is useful for in vitro blocking of target-specific CTL-mediated cell lysis1, as well as T cell activation assays, inducing proliferation and cytokine production1,2,7,12,16. It also induces apoptosis in immature thymocytes32,  and in vivo T cell depletion8-10. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1, immunohistochemical staining14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting4, complement-mediated cytotoxicity6, in vitro and in vivo stimulation of T cells1,2,7,12,16, immunofluorescent staining5, and in vivo T cell depletion8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells11. Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Leo O, et al. 1987. P. Natl. Acad. Sci. USA 84:1374. (IP, Activ, Block)
  2. Kruisbeek AM, et al. 1991. In Current Protocols in Immunology. 3.12.1. (Activ)
  3. Duke RC, et al. 1995. Current Protocols in Immunology. 3.17.1.
  4. Salvadori S, et al. 1994. J. Immunol. 153:5176. (WB)
  5. Payer E, et al. 1991. J. Immunol. 146:2536. (IF)
  6. Jacobs H, et al. 1994. Eur. J. Immunol. 24:934. (CMCD)
  7. Vossen ACTM, et al. 1995. Eur. J. Immunol. 25:1492. (Activ)
  8. Henrickson M, et al. 1995. Transplantation 60:828. (Deplete)
  9. Kinnaert P, et al. 1996. Transpl. Int. 9:386. (Deplete)
  10. Han WR, et al. 1999. Transpl. Immunol. 7:207. (Deplete)
  11. Miescher GC, et al. 1989. Immunol. Lett. 23:113. (Block)
  12. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Activ)
  13. Ko SY, et al. 2005. J. Immunol. 175:3309.
  14. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC-F)
  15. Tilley SL, et al. 2007. J. Immunol. 178:3208. (IHC-F)
  16. Wang W, et al. 2007. J. Immunol. 178:4885. (Activ)
  17. Xiao S, et al. 2007. J. Exp. Med. 204:1691.
  18. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed.
  19. Curtsinger JM, et al.2005. J. Immunol. 175:4392. PubMed
  20. Guo Y, et al. 2008. Blood 112:480. PubMed
  21. Kenna TJ, et al. 2008. Blood 111:2091.
  22. Perchonock CE, et al. 2007. J. Immunol. 179:1768. PubMed
  23. Perchonock GE, et al. 2006. Mol. Cell. Biol. 26:6005. PubMed
  24. Kanaya T, et al. 2008. Am. J. Physiol. Gastrointest. Liver Physiol. 295:G273. PubMed
  25. de Koning BA, et al. 2006. Int. Immunol. 18:941. PubMed
  26. Schulteis RD, et al. 2008. Blood 295:G273. PubMed
  27. Qi Q, et al. 2009. Blood 114:564. PubMed
  28. Helmersson S, et al. 2013. Am J Pathol. 9440:123. Pubmed
  29. Wu S, et al. 2014. Clin Vaccine Immunol. 21:156. PubMed
  30. Yan J, et al. 2014. Vaccine. 32:2833. PubMed
  31. Guiterrez DA, et al. 2014. Diaebetes. 63:3827. PubMed
  32. Shi YF, et al. 1991. J Immunol. 146:3340. (Apop)
Product Citations
  1. Kim O, et al. 2017. Nat Commun. 10.1038/s41467-017-00729-8. PubMed
  2. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  3. Johnson JL et al. 2018. Immunity. 48(2):243-257 . PubMed
  4. Logan K Smith et al. 2018. Immunity. 48(2):299-312 . PubMed
  5. Tanimura N et al. 2018. Developmental cell. 46(5):581-594 . PubMed
  6. Giambra V et al. 2018. Cell stem cell. 23(5):714-726 . PubMed
  7. Álvaro de Mingo Pulido et al. 2018. Cancer cell. 33(1):60-74 . PubMed
  8. Li Q et al. 2018. Immunity. 48(2):258-270 . PubMed
  9. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  10. Galle-Treger L, et al. 2019. Nat Commun. 10:713. PubMed
  11. Kimball A, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1102. PubMed
  12. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  13. Balzano M et al. 2019. Cell reports. 26(12):3257-3271 . PubMed
  14. Matsumura T et al. 2019. Cell Rep. 27(2):561-571 . PubMed
  15. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  16. Adachi Y, et al. 2019. Nat Commun. 10:3883. PubMed
  17. Onodera T, et al. 2019. J Immunol. 203:3282. PubMed
  18. Nagashima H et al. 2019. Immunity. 51(4):682-695 . PubMed
  19. Kimball AS et al. 2019. Immunity. 51(2):258-271 . PubMed
  20. Roco JA et al. 2019. Immunity. 51(2):337-350 . PubMed
  21. O'Boyle C, et al. 2020. Int J Stroke. 0.746527778. PubMed
  22. Park DS, et al. 2019. PLoS Comput Biol. 15:e1006913. PubMed
  23. Davis FM, et al. 2019. Arterioscler Thromb Vasc Biol. 39:2353. PubMed
  24. van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
  25. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  26. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
  27. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  28. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  29. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  30. Liao G, et al. 2010. Int Immunol. 1.096527778. PubMed
  31. Ho K, et al. 2014. Cell Death Dis. 5:1518. PubMed
  32. Honda H, et al. 2014. J Leukoc Biol. 96:1087. PubMed
  33. Muro R, et al. 2015. PLoS One. 10:119898. PubMed
  34. Suzuki M, et al. 2015. J Immunol. 195: 1273-1281. PubMed
  35. Matsuzaki Y, et al. 2015. Biomed Rep. 1: 91 - 97. PubMed
  36. Fournier M, Bijl C 2015. Stem Cells Dev. 24: 2413-2422. PubMed
  37. Pearce V, et al. 2015. J Immunol. 195: 3206 - 3217. PubMed
  38. Doisne J, et al. 2015. J Immunol. 195: 3937 - 3945.. PubMed
  39. Lutz J, et al. 2015. Nat Commun. 6: 8575. PubMed
  40. Lim H, et al. 2015. J Immunol. 195: 5432 - 5439. PubMed
  41. Olsson A, et al. 2016. Nature. 10.1038/nature19348. PubMed
  42. Kurkewich J, et al. 2016. PLoS One. 11: 0161468. PubMed
  43. Zysset D, et al. 2016. Nat Commun. 7:13151. PubMed
  44. Bieber K, et al. 2016. Sci Rep. 6:38357. PubMed
  45. Köchl R, et al. 2020. Elife. 9:00. PubMed
  46. Pariser DN, et al. 2021. J Clin Invest. 131:00:00. PubMed
  47. Kimball AS, et al. 2020. Thromb Haemost. 120:289. PubMed
  48. Kim S, et al. 2020. Immunity. 53(4):759-774.e9. PubMed
  49. Opejin A, et al. 2020. Cell Reports. 33(8):108424. PubMed
  50. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
  51. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  52. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  53. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  54. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  55. de Mingo Pulido , et al. 2021. Immunity. 54(6):1154-1167.e7. PubMed
  56. Riedel SS, et al. 2021. Molecular Cell. 81(11):2332-2348.e9. PubMed
  57. Bae S, et al. 2021. Cell Reports. 35(11):109264. PubMed
  58. Lu SX, et al. 2021. Cell. . PubMed
  59. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  60. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  61. Davis FM, et al. 2020. J Immunol. 2503:204. PubMed
  62. Li ZG, et al. 2020. Chin Med J (Engl). 1943:133. PubMed
  63. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  64. Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed
  65. Perez E, et al. 2020. J Immunol. 205:2489. PubMed
  66. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  67. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  68. Onodera T, et al. 2021. Immunity. 54:2385. PubMed
  69. Hinterbrandner M, et al. 2021. JCI Insight. 6:e151797. PubMed
  70. Galle-Treger L, et al. 2020. J Allergy Clin Immunol. 145:502. PubMed
  71. Wuggenig P, et al. 2020. Commun Biol. 3:130. PubMed
  72. Davis FM, et al. 2021. J Exp Med. 218:. PubMed
  73. Klarquist J, et al. 2021. Cell Rep. 36:109591. PubMed
  74. Koide S, et al. 2022. iScience. 25:103603. PubMed
  75. Progatzky F, et al. 2021. Nature. 599:125. PubMed
  76. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  77. Celias DP, et al. 2022. STAR Protoc. 3:101464. PubMed
  78. Kotaki R, et al. 2020. Sci Rep. 10:13554. PubMed
  79. She L, et al. 2021. JCI Insight. 6:e143509. PubMed
  80. Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed
  81. Blanchard A, et al. 2021. Nat Commun. 12:4693. PubMed
  82. Deets KA, et al. 2021. Elife. 10:. PubMed
  83. Anderson DA, et al. 2022. J Exp Med. 219:. PubMed
  84. Sasse C, et al. 2022. Pathogens. 11:. PubMed
  85. Murata T, et al. 2020. Sci Rep. 10:13560. PubMed
  86. Lin YH, et al. 2021. Front Immunol. 12:626418. PubMed
  87. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  88. Wolf SJ, et al. 2022. JCI Insight. 7:. PubMed
  89. Wagner AK, et al. 2022. iScience. 25:105137. PubMed
  90. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  91. Song R, et al. 2022. Immunology. 165:274. PubMed
  92. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  93. Merana GR, et al. 2022. Cell Rep. 39:110891. PubMed
  94. Gupta S, et al. 2022. Sci Adv. 8:eabn4886. PubMed
  95. Koizumi M, et al. 2022. J Biol Chem. 298:102506. PubMed
  96. Tomiaki C, et al. 2022. Front Immunol. 13:1014462. PubMed
  97. Giannou AD, et al. 2023. Immunity. 56:125. PubMed
  98. Gonçalves R, et al. 2023. iScience. 26:105972. PubMed
  99. De Giovanni M, et al. 2022. Cell. 185:815. PubMed
  100. Obi AT, et al. 2023. Sci Rep. 13:3253. PubMed
  101. Miyake K, et al. 2023. Nat Commun. 14:2694. PubMed
RRID
AB_312668 (BioLegend Cat. No. 100303)
AB_312669 (BioLegend Cat. No. 100304)

Antigen Details

Structure
Ig superfamily, forms CD3/TCR complex with CD3δ, γ and ζ subunits and TCR (α/β and γ/δ), 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
TCR signal transduction, T cell activation, antigen recognition
Ligand/Receptor
Peptide antigen/MHC-complex
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12501 View all products for this Gene ID
UniProt
View information about CD3epsilon on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11/30/2012

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account