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Brilliant Violet 421™ anti-human CD14 Antibody

Pricing & Availability
Clone
HCD14 (See other available formats)
Regulatory Status
RUO
Other Names
LPS receptor
Isotype
Mouse IgG1, κ
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Product Citations
publications
HCD14_BV421_040711
Human peripheral blood monocytes were stained with CD14 (clone HCD14) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
  • HCD14_BV421_040711
    Human peripheral blood monocytes were stained with CD14 (clone HCD14) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
See Brilliant Violet 421™ spectral data
Cat # Size Price Save
325627 25 tests ¥43,560
325628 100 tests ¥88,440
Description

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.

Application References

(PubMed link indicates BioLegend citation)
  1. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
  2. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Product Citations
  1. Joly P, et al. 2017. PLoS One. 12(6):e0180568. PubMed
  2. Hilt ZT, et al. 2019. JCI Insight. 4:5. PubMed
  3. Burel JG, et al. 2019. Elife. 8. PubMed
  4. Zhichao Fan et al. 2019. Cell reports. 26(1):119-130 . PubMed
  5. Dos Santos JC, et al. 2019. Cell Rep. 28:2659. PubMed
  6. Forconi CS, et al. 2020. Frontiers in Cellular and Infection Microbiology. 0.529166667. PubMed
  7. Cirovic B, et al. 2020. Cell Host & Microbe. 28(2):322-334. PubMed
  8. Zhong J, et al. 2018. Sci Adv. 4:eaas9864. PubMed
  9. Fan Z, et al. 2020. STAR Protoc. 1:100012. PubMed
  10. Frei A, et al. 2016. Nat Methods. 10.1038/nmeth.3742. PubMed
  11. Watson D, et al. 2016. Biomaterials. 105:195-205. PubMed
  12. Holokai L, et al. 2020. Cancers (Basel). 12:00. PubMed
  13. Qiu J, et al. 2020. Cell Rep. 33:108465. PubMed
  14. Walsh RE, et al. 2020. MAbs. 12:1764829. PubMed
  15. Jost M, et al. 2021. eLife. 10:00. PubMed
  16. Caduff N, et al. 2021. Cell Reports. 35(5):109056. PubMed
  17. Richert-Spuhler LE, et al. 2021. Cell Reports Medicine. 2(6):100322. PubMed
  18. Gamradt S, et al. 2021. iScience. 24:103312. PubMed
  19. Ibidapo-Obe O, et al. 2020. Cell Mol Gastroenterol Hepatol. 9:661. PubMed
  20. Forsberg MH, et al. 2021. Stem Cell Res Ther. 12:459. PubMed
  21. Hegewisch-Solloa E, et al. 2021. J Immunol. 207:950. PubMed
  22. Feng S, et al. 2022. Front Immunol. 13:990522. PubMed
  23. Sulaj A, et al. 2022. J Clin Endocrinol Metab. 107:2167. PubMed
  24. Pioch J, et al. 2022. J Immunol Methods. 507:113308. PubMed
  25. Varun K, et al. 2023. EBioMedicine. 90:104516. PubMed
  26. Toriyama M, et al. 2023. Front Mol Biosci. 10:1149828. PubMed
RRID
AB_2561342 (BioLegend Cat. No. 325627)
AB_2563296 (BioLegend Cat. No. 325628)

Antigen Details

Structure
GPI-linked membrane glycoprotein, 53-55 kD
Distribution

Monocytes, macrophages, granulocytes (low)

Function
LPS receptor, clearance of Gram-negative pathogens
Ligand/Receptor
LPS
Cell Type
Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434.

Gene ID
929 View all products for this Gene ID
UniProt
View information about CD14 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 2    Revision Date: 07/13/2015

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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