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Brilliant Violet 421™ anti-human CD34 Antibody

Pricing & Availability
Clone
561 (See other available formats)
Regulatory Status
RUO
Other Names
Gp105-120, My10, Mucosialin
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
561_BV421_1_021612
Human peripheral blood mononuclear cells were stained with CD14 PE, CD45 PerCP/Cy5.5, and CD34 (clone 561) Brilliant Violet 421™ (top) or mouse IgG2a Brilliant Violet 421™ isotype control (bottom). Dot plots are from gated on CD14-negative cell population.
  • 561_BV421_1_021612
    Human peripheral blood mononuclear cells were stained with CD14 PE, CD45 PerCP/Cy5.5, and CD34 (clone 561) Brilliant Violet 421™ (top) or mouse IgG2a Brilliant Violet 421™ isotype control (bottom). Dot plots are from gated on CD14-negative cell population.
  • 561_BV421_2_021612
See Brilliant Violet 421™ spectral data
Cat # Size Price Save
343609 25 tests ¥44,880
343610 100 tests ¥88,440
Description

CD34, also known as gp105-120, is a type I monomeric sialomucin-like glyco-phosphoprotein with an approximate molecular weight of 105-120 kD. It is selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells. Based on different sensitivities, four groups of epitopes of CD34 have been described. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The 561 antibody recognizes a class III group epitope, which is resistant to sialidase/glycolyprotease and chymopapain treatment.

Application References

(PubMed link indicates BioLegend citation)
  1. Croockewit AJ, et al. 1998. Scand. J. Immunol. 47:82.
  2. Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36.
Product Citations
  1. Seo GY et al. 2018. Cell host & microbe. 24(2):249-260 . PubMed
  2. Osorio FG et al. 2018. Cell reports. 25(9):2308-2316 . PubMed
  3. Lu YC, et al. 2018. Cell Rep. 25:2083. PubMed
  4. Crippa S, et al. 2019. J Clin Invest. 129:1566. PubMed
  5. Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed
  6. Nafria M, et al. 2020. Cell Rep. 31:107691. PubMed
  7. Ward D, et al. 2016. Haematologica. 101: 286 - 296. PubMed
  8. Serr I, et al. 2016. Nat Commun. 7:10991. PubMed
  9. Vasu S, et al. 2016. Blood. 127: 2879 - 2889. PubMed
  10. Nafria M, et al. 2020. STAR Protoc. 1:100130. PubMed
  11. Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
  12. Tong J, et al. 2021. Cell Stem Cell. 28(3):502-513.e6. PubMed
  13. Brandsma AM, et al. 2021. Cancer Discov. . PubMed
  14. Brandsma AM, et al. 2021. Blood Cancer Discov. 2:484. PubMed
  15. Meng YM, et al. 2021. Nat Commun. 12:6011. PubMed
  16. Hasaart KAL, et al. 2022. iScience. 25:103736. PubMed
  17. Rosendahl Huber A, et al. 2022. STAR Protoc. 3:101361. PubMed
  18. de Kanter JK, et al. 2021. Cell Stem Cell. 28:1726. PubMed
  19. Schielke L, et al. 2022. Front Immunol. 13:916701. PubMed
  20. Evans WS, et al. 2020. Exp Physiol. 105:1408. PubMed
  21. Yuzuriha A, et al. 2021. Methods Mol Biol. 2454:411. PubMed
  22. Rabold K, et al. 2022. Nat Commun. 13:6149. PubMed
  23. Ainsworth RI, et al. 2022. Nat Commun. 13:6221. PubMed
  24. Scala S, et al. 2023. Nat Commun. 14:3068. PubMed
RRID
AB_11147951 (BioLegend Cat. No. 343609)
AB_2561358 (BioLegend Cat. No. 343610)

Antigen Details

Structure
105-120 kD single chain mucin-like glycoprotein
Distribution

Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts

Function
Cell adhesion
Ligand/Receptor
L-selecin, E-selectin
Cell Type
Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family
CD Molecules
Gene ID
947 View all products for this Gene ID
UniProt
View information about CD34 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 2    Revision Date: 11/01/2016

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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