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Brilliant Violet 421™ anti-human CD38 Antibody

Pricing & Availability
Clone
HIT2 (See other available formats)
Regulatory Status
RUO
Workshop
III 155
Other Names
T10, ADP-ribosyl cyclase
Isotype
Mouse IgG1, κ
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Product Citations
publications
HIT2_BV421_032911
Human peripheral blood lymphocytes were stained with CD38 (clone HIT2) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
  • HIT2_BV421_032911
    Human peripheral blood lymphocytes were stained with CD38 (clone HIT2) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
See Brilliant Violet 421™ spectral data
Cat # Size Price Save
303525 25 tests ¥33,820
303526 100 tests ¥67,820
Description

CD38 is a 45 kD type II transmembrane glycoprotein also known as T10. It is an ADP-ribosyl hydrolase expressed at variable levels on hematopoietic cells and in some non-hematopoietic tissues (such as brain, muscles, and kidney). In humans, it is expressed at high levels on plasma cells and activated T and B cells. By functioning as both a cyclase and a hydrolase, CD38 mediates lymphocyte activation, adhesion, and the metabolism of cADPR and NAADP. CD31 is the ligand of CD38.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6 and spatial biology (IBEX)10,11.

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  2. Dieu M. 1998. J. Exp. Med. 188:373.
  3. Esser M, et al. 2001. J. Virol. 75:6173.
  4. Jeannin P, et al. 1999. J. Immunol. 162:2044.
  5. Kapsogeorgou EK, et al. 2001. J. Immunol. 166:3107.
  6. van der Voort R, et al. 1997. J. Exp. Med. 185:2121. (IHC)
  7. Bende RJ, et al. 2003. Am. J. Pathol. 162:105.
  8. Lehner M, et al. 2008. J. Leukoc. Biol. 83:883. PubMed
  9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  10. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Kamekura R,et al. 2017. J Immunol. 10.4049/jimmunol.1601507. PubMed
  2. Garfall AL, et al. 2018. JCI Insight. 3. PubMed
  3. Floros VI, et al. 2018. Nat Cell Biol. 20:144. PubMed
  4. Hill DL, et al. 2019. J Exp Med. 216:1857. PubMed
  5. Christiansen M, et al. 2020. Front Immunol. 10:3022. PubMed
  6. Lee J, et al. 2015. J Exp Med. 212:385. PubMed
  7. Breton G, et al. 2015. J Exp Med. 212:401. PubMed
  8. Montezuma-Rusca J, et al. 2015. J Immunol. 194:2561. PubMed
  9. Inui M, et al. 2015. Int Immunol. 27: 345 - 355. PubMed
  10. An G, et al. 2016. Blood. 128: 1590 - 1603. PubMed
  11. Woda M, et al. 2016. J Infect Dis. 214: 1001 - 1009. PubMed
  12. Inui M, et al. 2016. Int Immunol. 28(12):597-604. PubMed
  13. Stunnenberg M, et al. 2020. Viruses. 12:00. PubMed
  14. Ishibashi M, et al. 2020. Mol Cancer Res. 632:18. PubMed
  15. Sakharkar M, et al. 2021. Sci Immunol. :6. PubMed
  16. Cohen CA, et al. 2021. Nat Commun. 12:4678. PubMed
  17. Domenger A, et al. 2022. EMBO Mol Med. 14:e14740. PubMed
  18. Wang X, et al. 2022. J Exp Clin Cancer Res. 41:210. PubMed
  19. Turner JS, et al. 2020. Nature. 586:127. PubMed
  20. Schaefer-Babajew D, et al. 2023. Nature. 613:735. PubMed
RRID
AB_10900230 (BioLegend Cat. No. 303525)
AB_10983072 (BioLegend Cat. No. 303526)

Antigen Details

Structure
ADP-ribosyl cyclase, ectoenzyme, type II glycoprotein, 45 kD
Distribution

T cells, B cells, NK, myeloid, plasma, and dendritic cells

Function
Ecto-ADP-ribosyl cyclase, calcium signaling, cell activation
Ligand/Receptor
CD31, hyaluronic acid
Cell Type
B cells, Dendritic cells, NK cells, Plasma cells, T cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Ferrero E, et al. 1999. J. Leukoc. Biol. 65:151.
2. Lund F, et al. 1995. Immunol. Today 16:469.

Gene ID
952 View all products for this Gene ID
UniProt
View information about CD38 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 2    Revision Date: 12/17/2021

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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