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Brilliant Violet 421™ anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Regulatory Status
RUO
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
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Product Citations
publications
17A2_BV421_1_062911
C57BL/6 mouse splenocytes were stained with CD19 APC and CD3 (clone 17A2) Brilliant Violet 421™ (top) or rat IgG2b, κ Brilliant Violet 421™ isotype control (bottom).
  • 17A2_BV421_1_062911
    C57BL/6 mouse splenocytes were stained with CD19 APC and CD3 (clone 17A2) Brilliant Violet 421™ (top) or rat IgG2b, κ Brilliant Violet 421™ isotype control (bottom).
  • 17A2_BV421_2_062911
  • 17A2_BV421_CD3_Antibody_IF_012914
    C57BL/6 mouse splenocytes were fixed with 2% paraformaldehyde (PFA), and then stained with 5 µg/ml CD3 (clone 17A2) Brilliant Violet 421™ (blue) and 5 µg/ml CD19 (clone 6D5) Alexa Fluor® 647 (red) for 30 minutes at room temperature. The image was captured by 40X objective.
See Brilliant Violet 421™ spectral data
Cat # Size Price Save
100227 125 µL ¥38,720
100228 50 µg ¥51,730
Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunocytochemistry using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.

Application References

(PubMed link indicates BioLegend citation)
  1. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Emgård J, et al. 2018. Immunity. 143:419. PubMed
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  3. Jenkins RW, et al. 2018. Cancer Discov. 8:196. PubMed
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  5. Britton GJ et al. 2019. Immunity. 50(1):212-224 . PubMed
  6. Jackstadt R, et al. 2019. Cancer Cell. 36:319. PubMed
  7. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  8. Telford W, et al. 2017. Cytometry A. 91:314. PubMed
  9. Zhu D, et al. 2017. Stem Cell Res Ther. 0.511805556. PubMed
  10. Hu Z, et al. 2020. PLoS One. 15:e0228339. PubMed
  11. Royzman D, et al. 2019. Front Immunol. 10:633. PubMed
  12. Fleskens V, et al. 2019. Cell Rep. 26:3600. PubMed
  13. Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed
  14. Jin C, et al. 2019. Cell. 176:998. PubMed
  15. John S, et al. 2020. Sci Rep. 10:11377. PubMed
  16. Lee H, et al. 2020. Cell Metabolism. 31(4):822-836. PubMed
  17. Chao CC, et al. 2020. Cell. 179(7):1483-1498.e22.. PubMed
  18. Chukkapalli S, et al. 2015. Pathog Dis. 73:9. PubMed
  19. Cameron G, et al. 2015. J Immunol. 195: 4604 - 4614. PubMed
  20. Chukkapalli S, et al. 2015. PLoS One. 10: 0143291. PubMed
  21. Solaymani-Mohammadi S, et al. 2016. J Leukoc Biol. 99: 475 - 482. PubMed
  22. Hasegawa T, et al. 2020. Sci Rep. 10:13480. PubMed
  23. Elahi S, et al. 2020. Stem Cell Res. 43:101710. PubMed
  24. Bertocchi A, et al. 2021. Cancer Cell. 39(5):708-724.e11. PubMed
  25. Chow AK, et al. 2021. Cellular and Molecular Gastroenterology and Hepatology. :. PubMed
  26. Cartwright ANR, et al. 2021. Cancer Immunol Res. 9:470. PubMed
  27. Kalinina A, et al. 2021. STAR Protoc. 2:100368. PubMed
  28. Trenevska I, et al. 2021. PLoS One. 16:e0249967. PubMed
  29. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
  30. Henkle TR, et al. 2021. Cancer Res. 81:4560. PubMed
  31. Montes de Oca R, et al. 2021. Mol Cancer Ther. 20:1941. PubMed
  32. Almshayakhchi R, et al. 2021. Front Oncol. 11:636977. PubMed
  33. Chen G, et al. 2021. Sci Adv. 7:eabg5686. PubMed
  34. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  35. Heil J, et al. 2021. Nat Commun. 12:6963. PubMed
  36. Tummala G, et al. 2021. Invest Ophthalmol Vis Sci. 62:41. PubMed
  37. Bristow CL, et al. 2021. Front Oncol. 11:739080. PubMed
  38. Schädlich IS, et al. 2022. iScience. 25:104470. PubMed
  39. Royzman D, et al. 2022. Front Immunol. 13:1012647. PubMed
  40. Yu Y, et al. 2022. Nat Commun. 13:6357. PubMed
  41. Chen J, et al. 2022. Nat Commun. 13:6759. PubMed
  42. Mills EL, et al. 2022. Cell Metab. 34:140. PubMed
  43. Kiani Shabestari S, et al. 2022. Cell Rep. 39:110961. PubMed
  44. Jin SM, et al. 2023. Nat Nanotechnol. :. PubMed
  45. Oh S, et al. 2023. Nat Biotechnol. :. PubMed
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  47. Sanmarco LM, et al. 2022. Nature. 611:801. PubMed
  48. Liu H, et al. 2023. Breast Cancer Res. 25:43. PubMed
  49. Bhaskar A, et al. 2023. iScience. 26:106644. PubMed
RRID
AB_10900227 (BioLegend Cat. No. 100227)
AB_2562553 (BioLegend Cat. No. 100228)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 3    Revision Date: 01/04/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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