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Brilliant Violet 510™ anti-human CD8a Antibody

Pricing & Availability
Clone
RPA-T8 (See other available formats)
Regulatory Status
RUO
Workshop
IV T171
Other Names
T8, Leu2
Isotype
Mouse IgG1, κ
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Product Citations
publications
RPA-T8_BV510_CD8_Antibody_FC_082512
Human peripheral blood lymphocytes were stained with CD3 FITC and CD8 (clone RPA-T8) Brilliant Violet 510™.
  • RPA-T8_BV510_CD8_Antibody_FC_082512
    Human peripheral blood lymphocytes were stained with CD3 FITC and CD8 (clone RPA-T8) Brilliant Violet 510™.
See Brilliant Violet 510™ spectral data
Cat # Size Price Save
301047 25 tests ¥38,720
301048 100 tests ¥67,640
Description

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation, and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Product Details
Technical data sheet

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The RPA-T8 antibody does not block the binding of HIT8a antibody to CD8a. Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed frozen sections3 and costimulation of T cell responses4. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 301073 & 301074).

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  3. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  4. Magidovich E, et al. 2007. P. Natl. Acad. Sci. USA 104:13022.
  5. Thakarl D, et al. 2008.J. immunol. 180:7431. PubMed
  6. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  7. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
  10. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
Product Citations
  1. Carnevale G,et al. 2017. Sci Rep.. 10.1038/s41598-017-14838-3. PubMed
  2. Beraud C, et al. 2018. Virol J. 15:35. PubMed
  3. Collinson–Pautz MR, et al. 2019. Leukemia. 33:2195. PubMed
  4. Moguche AO et al. 2017. Cell host & microbe. 21(6):695-706 . PubMed
  5. Telford W, et al. 2017. Cytometry A. 91:314. PubMed
  6. Iwahori K, et al. 2019. Sci Rep. 2.205555556. PubMed
  7. Fong L, et al. 2019. Cancer Discov. 10:40. PubMed
  8. Mothe B, et al. 2020. Front Immunol. 1.029861111. PubMed
  9. Aliu B, et al. 2020. Journal of Neurochemistry. 154(5):486-501. PubMed
  10. Bennstein SB, et al. 2020. eLife. 9:e55232.. PubMed
  11. Severson J, et al. 2015. Cancer Immunol Res. 3:620. PubMed
  12. Hammitzsch A, et al. 2015. Proc Natl Acad Sci U S A. 112: 10768-10773. PubMed
  13. Wheatley A, et al. 2016. Sci Rep. 6: 26478. PubMed
  14. Kariminia A, et al. 2016. Blood. 127: 3082 - 3091. PubMed
  15. Bruno L, et al. 2016. Immunol Cell Biol. 10.1038/icb.2016.49. PubMed
  16. Chandele A, et al. 2016. J Virol. 90(24):11259-11278. PubMed
  17. Juno JA, et al. 2020. Nat Med. 26:1428. PubMed
  18. Eslamizar L, et al. 2021. NPJ Vaccines. 6:15. PubMed
  19. Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed
  20. Litchfield K, et al. 2021. Cell. 184(3):596-614.e14. PubMed
  21. Sutton HJ, et al. 2021. Cell Reports. 34(6):108684. PubMed
  22. Townsley SM, et al. 2021. Cell Host Microbe. 29(4):564-578.e9. PubMed
  23. Mukhamedova M, et al. 2021. Immunity. 54(4):769-780.e6. PubMed
  24. Punik J, et al. 2021. Cell Reports. 35(13):109320. PubMed
  25. Townsley SM, et al. 2021. STAR Protocols. 2(3):100771. PubMed
  26. Nguyen THO, et al. 2021. Immunity. 54:1066. PubMed
  27. Tan J, et al. 2021. Sci Transl Med. 13: . PubMed
  28. Duhen R, et al. 2021. Nat Commun. 12:1047. PubMed
  29. Lu Y, et al. 2021. Gastroenterology. 161:575. PubMed
  30. Wang L, et al. 2021. Science. 373:. PubMed
  31. Nakano M, et al. 2021. Front Immunol. 12:713225. PubMed
  32. Cho JW, et al. 2021. Nat Commun. 12:6068. PubMed
  33. Keeton R, et al. 2021. Cell Host Microbe. 29:1611. PubMed
  34. Moreno-Fernandez ME, et al. 2021. STAR Protoc. 2:100937. PubMed
  35. Ma KY, et al. 2021. Nat Immunol. 22:1590. PubMed
  36. Yang L, et al. 2020. Genes Dis. 7:128. PubMed
  37. Haruna M, et al. 2020. Biol Pharm Bull. 43:399. PubMed
  38. Port JR, et al. 2020. J Virol. 94:. PubMed
  39. Rajamanickam V, et al. 2021. Cancer Immunol Res. 9:602. PubMed
  40. Wheatley AK, et al. 2021. Cell Rep. 37:109822. PubMed
  41. Liisborg C, et al. 2022. PLoS One. 17:e0269960. PubMed
  42. Silver J, et al. 2020. Methods Mol Biol. 2121:115. PubMed
  43. Ellis GI, et al. 2022. STAR Protoc. 3:101784. PubMed
  44. Ellis GI, et al. 2022. Cell Rep Med. 3:100614. PubMed
  45. Sudmeier LJ, et al. 2022. Cell Rep Med. 3:100620. PubMed
  46. Koutsakos M, et al. 2022. Immunity. 55:1316. PubMed
  47. Lima NS, et al. 2022. Nat Commun. 13:7733. PubMed
  48. Keeton R 2023. Cell Reports Medicine. 4(1):100898. PubMed
  49. Du Bruyn E, et al. 2023. Nat Commun. 14:188. PubMed
  50. Zhang B, et al. 2023. Signal Transduct Target Ther. 8:28. PubMed
  51. Rosain J, et al. 2023. Cell. 186:621. PubMed
  52. Liisborg C, et al. 2022. Acta Ophthalmol. :3. PubMed
  53. Pinto C, et al. 2023. iScience. 26:106381. PubMed
  54. Saotome K, et al. 2023. Nat Commun. 14:2401. PubMed
RRID
AB_2561378 (BioLegend Cat. No. 301047)
AB_2561942 (BioLegend Cat. No. 301048)

Antigen Details

Structure
Ig superfamily, homodimer or heterodimer with CD8β, 32-34 kD
Distribution

Majority of thymocytes, T cell subset, NK cells

Function
MHC class I co-receptor, thymic differentiation, T cell activation
Ligand/Receptor
MHC Class I molecules
Cell Type
Dendritic cells, NK cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.

Gene ID
925 View all products for this Gene ID
UniProt
View information about CD8alpha on UniProt.org
Go To Top Version: 3    Revision Date: 07/13/2015

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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