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FITC anti-human CD73 (Ecto-5'-nucleotidase) Antibody

Pricing & Availability
Clone
AD2 (See other available formats)
Regulatory Status
RUO
Workshop
V B-CD73.3
Other Names
Ecto-5'-nucleotidase, E.C3.1.3.5, L-VAP-2, NT5E, 5'-NT
Isotype
Mouse IgG1, κ
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Product Citations
publications
A_AD2_FITC_CD73_Antibody_FC_1_020713
Human peripheral blood lymphocytes were stained with CD3 APC and CD73 (clone AD2) FITC (top) or mouse IgG1, κ FITC isotype control (bottom).
  • A_AD2_FITC_CD73_Antibody_FC_1_020713
    Human peripheral blood lymphocytes were stained with CD3 APC and CD73 (clone AD2) FITC (top) or mouse IgG1, κ FITC isotype control (bottom).
  • B_AD2_FITC_CD73_Antibody_FC_2_020713
See FITC spectral data
Cat # Size Price Save
344015 25 tests ¥24,640
344016 100 tests ¥44,840
Description

CD73 is a 70 kD glycophosphatidylinositol (GPI)-linked 5'-nucleotidase, which is also known as ecto-5'-nucleotidase. It converts adenosine monophosphate (AMP) to adenosine. CD73 is expressed on subsets of T and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells. It has been reported that CD73 costimulates T cell activation, and mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include:immunofluorescence3.

Clone AD2 has been noted to induce clustering and internalization of CD73 in vivo and inhibit metastasis in a murine breast cancer xenograft model4.

Application References

(PubMed link indicates BioLegend citation)
  1. Nakamura T, et al. 1993. J. Immunol. 151:6933.
  2. Liao J, et al. 2011. J Endod. 37:1217. PubMed
  3. Touboul C, et al. 2013. J. Transl. Med. 11:28. (IF)
  4. Terp MG, et al. 2013. J Immunol. 191: 4165-73 (Block)
Product Citations
  1. Sun W, et al. 2019. Exp Ther Med. 17:2199. PubMed
  2. Gao P, et al. 2017. Cytotechnology. 69:751. PubMed
  3. Sprouse ML, et al. 2019. Int J Mol Sci. 20:8. PubMed
  4. Vishnoi M, et al. 2019. Mol Oncol. 13(9): 1913. PubMed
  5. Vishnoi M, et al. 2018. Cancer Res. 78:5349. PubMed
  6. Liu X, et al. 2020. Exp Ther Med. 1.258333333. PubMed
  7. He Z, et al. 2020. J Exp Clin Cancer Res. 39:140. PubMed
  8. Morad G, et al. 2020. International Journal of Molecular Sciences. 21(11):3851. PubMed
  9. Yu C, et al. 2020. Sci Rep. 10:14521. PubMed
  10. Boucher JM, et al. 2018. Cardiovasc Drugs Ther. 32:519. PubMed
  11. Goodnough LH, et al. 2020. JBMR Plus. 4:e10398. PubMed
  12. Aomatsu E, et al. 2014. Sci Rep. 4:3652. PubMed
  13. Yusa K, et al. 2016. Sci Rep. 6:29462. PubMed
  14. Ambrosi TH, et al. 2020. Aging Cell. 19:e13164. PubMed
  15. Festag J, et al. 2020. Mol Ther Nucleic Acids. 1.330555556. PubMed
  16. Ludwig N, et al. 2020. Angiogenesis. 1.374305556. PubMed
  17. Ayhan S, et al. 2021. Journal of Cell Science. 134(6):. PubMed
  18. Tu S, et al. 2020. Cellular Signalling. 73:109695. PubMed
  19. Keshtkar S, et al. 2021. Stem Cells Int. 8857457:2020. PubMed
  20. Zheng C, et al. 2021. J Extracell Vesicles. 10:e12109. PubMed
  21. Zhang Q, et al. 2021. Bone Joint Res. 10:226. PubMed
  22. Yerneni SS, et al. 2021. MBio. 12:e0165721. PubMed
  23. Fei D, et al. 2021. Front Cell Dev Biol. 9:687258. PubMed
  24. Pauken CM, et al. 2021. Cancers (Basel). 13:. PubMed
  25. Liu Y, et al. 2022. iScience. 25:104405. PubMed
  26. Han J, et al. 2022. FEBS J. 289:417. PubMed
  27. Yan K, et al. 2022. iScience. 25:104822. PubMed
  28. Huang J, et al. 2022. Clin Exp Pharmacol Physiol. 49:881. PubMed
  29. Zhang T, et al. 2022. IUBMB Life. 74:1081. PubMed
  30. Xia KS, et al. 2023. Bioact Mater. 21:69. PubMed
  31. Wang H, et al. 2022. Oxid Med Cell Longev. 2022:3082969. PubMed
  32. Tevlin R, et al. 2023. Sci Rep. 13:2097. PubMed
  33. Aru B, et al. 2023. Balkan Med J. 40:117. PubMed
  34. Chen X, et al. 2023. Exp Ther Med. 25:99. PubMed
  35. Lee CW, et al. 2022. J Adv Res. 39:73. PubMed
  36. Bowley TY, et al. 2022. Cancer Res Commun. 2:1436. PubMed
  37. Lu Y, et al. 2023. Sci Rep. 13:8479. PubMed
  38. Hoover MY, et al. 2023. Nat Protoc. . PubMed
RRID
AB_2561808 (BioLegend Cat. No. 344015)
AB_2561809 (BioLegend Cat. No. 344016)

Antigen Details

Structure
GPI-linked 5'-nucleotidase, 70 kD
Distribution

Subsets of T cells and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells

Function
Catalyses dephosphorylation of adenosine monophosphate, costimulates T cell activation, mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells
Cell Type
B cells, Dendritic cells, Endothelial cells, Epithelial cells, Mesenchymal Stem Cells, T cells, Tregs
Biology Area
Costimulatory Molecules, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Zola H, et al. 2007. Leukocyte and stromal Cell Molecules:the CD Markers. A John Wiley & Sons Inc, Publication.
2. Airas L and Jalkanen  S, et al. 1996. Blood 88:1755.
3. Gutensohn W, et al. 1995. Cell Immunol. 161:213.
4. Airas L, et al. 1995. J. Exp. Med. 182:1603.

Gene ID
4907 View all products for this Gene ID
UniProt
View information about CD73 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 04/09/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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