- Clone
- P84 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- SHPS-1, BIT, P84, PTPNS1, CD172 antigen-like family member A
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![P84_FITC_1_051012 P84_FITC_1_051012](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/P84_FITC_1_051012.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse bone marrow cells were stained with CD11b APC and CD172a (clone P84) FITC (top) or rat IgG1, κ FITC isotype control (bottom). Data shown was gated on the myeloid cell population. -
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Fixed whole mount mouse spleen was stained with FITC CD172a (red) (clone P84), Alexa Fluor® 647 CD11c (yellow) (clone N418), and PE CD11b (blue) (clone M1/70). Isotype controls at the same concentrations were used for the negative control. Cells were mounted in Prolong Gold and imaged with a Leica SP8 confocal. Image courtesy of Grzegorz Chodaczek and Zbigniew Mikulski at LIAI.
Cat # | Size | Price | Save |
---|---|---|---|
144005 | 25 µg | ¥17,980 | |
144006 | 100 µg | ¥30,940 |
CD172a, also known as SIRPα, is a type I transmembrane protein with one V-set Ig-like and two C-set Ig-like domains in the extracellular portion, and two ITIM motifs and a proline-rich region in the cytoplasmic tail. CD172a is expressed by monocytes, macrophages, myeloid cells, and neuronal tissue. The phosphorylation of SIRPα ITIMs induces the recruitment and activation of the tyrosine phosphatases PTPN6 and PTPN11, resulting in the negative regulation of several biological processes. The ligands of CD172a are CD47, SP-A, and SP-D.
Product DetailsProduct Details
- Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse brain membrane protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
IHC-F - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
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Additional reported applications (for the relevant formats) include: blocking SIRPa interaction with CD474, in vivo blocking of dendritic cell migration3, enhancing of macrophage phagocytosis2,4, immunohistochemical staining of cerebellum frozen sections1, immunoprecipitation2,4, and spatial biology (IBEX)5,6.
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Application References
(PubMed link indicates BioLegend citation) -
- Comu S, et al. 1997. J. Neurosci. 17:8702. (IHC)
- Gresham HD, et al. 2000. J. Exp. Med. 191:515. (IP)
- Fukunaga A, et al. 2004. J. Immunol. 172:4091. (Block)
- Oldenborg PA, et al. 2000. Science 288:2051. (Block, IP)
- Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
- RRID
-
AB_11204432 (BioLegend Cat. No. 144005)
AB_11204425 (BioLegend Cat. No. 144006)
Antigen Details
- Structure
- Type I transmembrane protein
- Distribution
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Monocytes, macrophages, myeloid cells, neuronal tissue
- Function
- Negative regulation of several biological processes
- Interaction
- PTPN6, PTPN11
- Ligand/Receptor
- CD47, SP-A, SP-D
- Cell Type
- Dendritic cells, Macrophages, Monocytes, Neutrophils
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Adhesion Molecules, CD Molecules, Protein Kinases/Phosphatase
- Antigen References
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1. Zhao XW, et al. 2011. P. Natl. Acad. Sci. USA 108:18342.
2. Verjan-Garcia N, et al. 2011. J. Immunol. 187:2268.
3. Sato-Hashimoto M, et al. 2011. J. Immunol. 187:291.
4. Raymond M, et al. 2010. Eur. J. Immunol. 40:3510. - Gene ID
- 19261 View all products for this Gene ID
- UniProt
- View information about CD172alpha on UniProt.org
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