- Clone
- 3G8 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- V NK80
- Other Names
- FcγRIII, Fc gamma receptor, Fc gamma receptor 3
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
CD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).
Product DetailsProduct Details
- Reactivity
- Human,Cynomolgus,Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human PMN cells
- Formulation
-
test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
µg sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. - Preparation
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
- Concentration
- µg sizes: 0.5 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.
For test size, the suggested use of this reagent for immunofluorescent staining is 5 µl per 106 cells in 100 µl volume.
For µg sizes, the suggested use of this reagent for immunofluorescent staining is ≤1.0 µg per 106 cells in 100 µl volume.
It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the FcγRIIa and FcγRIIIb receptors causing neutrophil activation and aggregation18. Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile.
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6, immunoprecipitation3, stimulation of NK cell proliferation4, blocking of phagocytosis5, and blocking of immunoglobulin binding to FcγRIII7,8. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302049, 302050, 302057, 302058). -
Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Edberg J, et al. 1997. J. Immunol. 159:3849. (IP)
- Hoshino S, et al. 1991. Blood 78:3232. (Stim)
- Tamm A, et al. 1996. Immunol. 157:1576. (Block)
- Da Silva DM, et al. 2001. Int. Immunol. 13:633. (IHC)
- Holl V, et al. 2004. J. Immunol. 173:6274. (Block)
- Hober D, et al. 2002. J. Gen. Virol. 83:2169. (Block)
- Brainard DM, et al. 2009. J. Virol. 83:7305. PubMed
- Smed-Sörensen A, et al. 2008. Blood 111:5037. (Block) PubMed
- Timmerman KL, et al. 2008. J. Leukoc. Biol. 84:1271. (FC) PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Rout N, et al. 2010. PLoS One 5:e9787. (FC)
- Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC)
- Boltz A, et al. 2011. J. Biol Chem. 286:21896. PubMed
- Wu Z, et al. 2013. J. Virol. 87:7717. PubMed
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- Vossebeld PJ, et al. 1997. Biochem J. 323:87-94 (Stim)
- Product Citations
- RRID
-
AB_492979 (BioLegend Cat. No. 302024)
AB_2104003 (BioLegend Cat. No. 302032)
AB_492978 (BioLegend Cat. No. 302021)
Antigen Details
- Structure
- Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD)
- Distribution
-
NK cells, activated monocytes, macrophages, neutrophils
- Function
- Low affinity IgG Fc receptor, phagocytosis, ADCC
- Ligand/Receptor
- Aggregated IgG, IgG-antigen complex
- Cell Type
- Dendritic cells, Macrophages, Monocytes, Neutrophils, NK cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Fc Receptors
- Antigen References
-
1. Fleit H, et al. 1982. P. Natl. Acad. Sci. USA 79:3275.
2. Stroncek D, et al. 1991. Blood 77:1572.
3. Wirthmueller U, et al. 1992. J. Exp. Med. 175:1381. - Gene ID
- 2214 View all products for this Gene ID
- UniProt
- View information about CD16 on UniProt.org
Related FAQs
- Is our human Trustain FcX™ (cat# 422302) compatible with anti human CD16, CD32 and CD64 clones 3G8, FUN-2 and 10.1 respectively?
-
Yes
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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