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PE anti-human CD16 Antibody

Pricing & Availability
Clone
3G8 (See other available formats)
Regulatory Status
RUO
Workshop
V NK80
Other Names
FcγRIII, Fc gamma receptor, Fc gamma receptor 3
Isotype
Mouse IgG1, κ
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Product Citations
publications
3G8_PE_061807
Human peripheral blood lymphocytes stained with 3G8 PE
  • 3G8_PE_061807
    Human peripheral blood lymphocytes stained with 3G8 PE
  • 3G8_PE_CD16_Antibody_042922
    Human peripheral blood was stained with CD16 (clone 3G8) PE (solid line) or mouse IgG1, κ PE isotype control (dashed line).

    Data was acquired on the Moxi Flow, exported, and processed using FlowJo software.
See PE spectral data
Cat # Size Price Save
302007 25 tests ¥24,200
302008 100 tests ¥45,570
302056 100 µg ¥53,680
Description

CD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).

Product Details
Technical data sheet

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human PMN cells
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the FcγRIIa and FcγRIIIb receptors causing neutrophil activation and aggregation18. Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile.

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6, immunoprecipitation3, stimulation of NK cell proliferation4, blocking of phagocytosis5, and blocking of immunoglobulin binding to FcγRIII7,8. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302049, 302050, 302057, 302058).

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  3. Edberg J, et al. 1997. J. Immunol. 159:3849. (IP)
  4. Hoshino S, et al. 1991. Blood 78:3232. (Stim)
  5. Tamm A, et al. 1996. Immunol. 157:1576. (Block)
  6. Da Silva DM, et al. 2001. Int. Immunol. 13:633. (IHC)
  7. Holl V, et al. 2004. J. Immunol. 173:6274. (Block)
  8. Hober D, et al. 2002. J. Gen. Virol. 83:2169. (Block)
  9. Brainard DM, et al. 2009. J. Virol. 83:7305. PubMed
  10. Smed-Sörensen A, et al. 2008. Blood 111:5037. (Block) PubMed
  11. Timmerman KL, et al. 2008. J. Leukoc. Biol. 84:1271. (FC) PubMed
  12. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  13. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
  14. Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC)
  15. Boltz A, et al. 2011. J. Biol Chem. 286:21896. PubMed
  16. Wu Z, et al. 2013. J. Virol. 87:7717. PubMed
  17. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  18. Vossebeld PJ, et al. 1997. Biochem J. 323:87-94 (Stim)
Product Citations
  1. Zheng Q, et al. 2017. Mol Oncol. 10.1002/1878-0261.12056. PubMed
  2. Azizi E et al. 2018. Cell. 174(5):1293-1308 e36. PubMed
  3. Yang X, et al. 2019. Mol Med Rep. 19:1083. PubMed
  4. Woolsey C et al. 2019. Cell Rep. 28(12):3032-3046 . PubMed
  5. Maharaj S, et al. 2018. Nitric Oxide. 72:41:00. PubMed
  6. Yeo L, et al. 2018. J Clin Invest. 128:3460. PubMed
  7. Ostendorf L, et al. 2019. Front Immunol. 2.309027778. PubMed
  8. Crifò B, et al. 2019. J Immunol. 202:1521. PubMed
  9. Hakimi AA, et al. 2019. Cancer Discov. 9:510. PubMed
  10. Teijeira á, et al. 2020. Immunity. 52(5):856-871. PubMed
  11. Urlaub D, et al. 2019. Arthritis Res Ther. 1.067361111. PubMed
  12. Siegers G, et al. 2011. PLoS One. 6:e16700. PubMed
  13. Nguyen D, et al. 2006. Exp Hematol. 34:728. PubMed
  14. Reichwald K, et al. 2014. PLoS One. 9:85793. PubMed
  15. Radom-Aizik S, et al. 2014. Brain Behav Immun. 39:121. PubMed
  16. Teirlinck A, et al. 2015. Infect Immun . 83: 3732-3739. PubMed
  17. Glaser K, et al. 2016. PLoS One. 11: 0146898. PubMed
  18. Parameswaran R, et al. 2016. Nat Commun. 7: 11154. PubMed
  19. Schrøder M, et al. 2016. PLoS One. 11: 0157387. PubMed
  20. Palamides P, et al. 2016. Dis Model Mech. 9: 985 - 997. PubMed
  21. Huang SSY, et al. 2021. Biology. 10(8):. PubMed
  22. Jankeel A, et al. 2020. J Virol. :94. PubMed
  23. Devalraju KP, et al. 2021. PLoS One. 16:e0257185. PubMed
  24. Afolabi LO, et al. 2021. Front Immunol. 12:701671. PubMed
  25. Song S, et al. 2021. Commun Biol. 4:1338. PubMed
  26. Manukyan G, et al. 2020. Auto Immun Highlights. 11:5. PubMed
  27. Zong D, et al. 2021. BMC Biol. 19:79. PubMed
  28. Pathania AS, et al. 2022. Mol Ther Oncolytics. 25:308. PubMed
  29. Murdock BJ, et al. 2021. Neurol Neuroimmunol Neuroinflamm. 8:. PubMed
  30. Subedi N, et al. 2021. Sci Rep. 11:17084. PubMed
  31. Kerdidani D, et al. 2022. J Exp Med. 219:. PubMed
  32. Karantanos T, et al. 2022. Sci Adv. 8:eabl8952. PubMed
  33. Wang T, et al. 2022. Front Immunol. 13:954121. PubMed
  34. Balan I, et al. 2022. Front Immunol. 13:940095. PubMed
  35. Drummer DJ, et al. 2022. Int J Exerc Sci. 15:686. PubMed
  36. Walle T, et al. 2022. Sci Adv. 8:eabh4050. PubMed
  37. LaSalle TJ, et al. 2022. Cell Rep Med. 3:100779. PubMed
  38. Subedi N, et al. 2022. Adv Biol (Weinh). :e2200207. PubMed
  39. Ismailova A, et al. 2023. Front Immunol. 14:1123344. PubMed
  40. Parasar P, et al. 2022. Am J Reprod Immunol. 88:e13614. PubMed
  41. Zhou J, et al. 2023. Front Endocrinol (Lausanne). 14:1069395. PubMed
  42. Zhang Y, et al. 2023. Heliyon. 9:e14823. PubMed
RRID
AB_314207 (BioLegend Cat. No. 302007)
AB_314208 (BioLegend Cat. No. 302008)
AB_2564139 (BioLegend Cat. No. 302056)

Antigen Details

Structure
Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD)
Distribution

NK cells, activated monocytes, macrophages, neutrophils

Function
Low affinity IgG Fc receptor, phagocytosis, ADCC
Ligand/Receptor
Aggregated IgG, IgG-antigen complex
Cell Type
Dendritic cells, Macrophages, Monocytes, Neutrophils, NK cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Antigen References

1. Fleit H, et al. 1982. P. Natl. Acad. Sci. USA 79:3275.
2. Stroncek D, et al. 1991. Blood 77:1572.
3. Wirthmueller U, et al. 1992. J. Exp. Med. 175:1381.

Gene ID
2214 View all products for this Gene ID
UniProt
View information about CD16 on UniProt.org

Related FAQs

Is our human Trustain FcX™ (cat# 422302) compatible with anti human CD16, CD32 and CD64 clones 3G8, FUN-2 and 10.1 respectively?

Yes

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 05/23/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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