- Clone
- 5E8/CXCR2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD182, CXCR2, CD128b, IL-8RB, Type II IL-8 receptor
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Human peripheral blood granulocytes stained with 5E8/CXCR2 PE
Cat # | Size | Price | Save |
---|---|---|---|
320705 | 25 tests | ¥26,840 | |
320706 | 100 tests | ¥58,960 |
CXCR2 is a 67-70 kD seven-transmembrane protein, also known as IL-8 receptor B (IL-8RB), CD182, and CD128b. It is a CXC chemokine receptor belongs to G protein-coupled receptor (GPCR) family. CXCR2 is expressed as homodimer or heterodimer with CXCR1 and found on granulocytes, NK cells, subset of T lymphocytes, mast cells, monocytes, endothelial cells, megakarocytes, and oligodendrocytes. CXCR2 mediates neutrophil activation and chemotaxis, megakaryocytic proliferation, and angiogenesis via binding its ligands including IL-8(CXCL8), NAP-2(CXCL7), GCP-2(CXCL6), and GRO-α,β,γ (CXCL1, CXCL2, CXCL3).
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human CXCR2 transfected L1.2 cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
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Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
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Additional reported applications (for the relevant formats) include: The 5E8/CXCR2 antibody is useful for immunofluorescent staining and flow cytometric analysis of CXCR2 expression.
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Application References
(PubMed link indicates BioLegend citation) -
- Kyriakakis E, et al. 2011. J Leukoc Biol. 90:929. PubMed.
- Product Citations
- RRID
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AB_439806 (BioLegend Cat. No. 320705)
AB_439807 (BioLegend Cat. No. 320706)
Antigen Details
- Structure
- Seven transmembrane spanning, G protein-coupled receptor (CPCR), 67-70 kD, homodier or teterodimer with CXCR1
- Distribution
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Granulocytes, NK cells, subset of T cells, monocytes, endothelial cells, oligodendrocytes
- Function
- Neutrophil activation, chemotaxis, angiogenesis
- Ligand/Receptor
- IL-8(CXCL8), NAP-2(CXCL7), GCP-2(CXCL6), GRO-& alpha;,ß, γ (CXCL1, CXCL2, CXCL3)
- Cell Type
- Granulocytes, NK cells, T cells, Monocytes, Endothelial cells, Oligodendrocytes, Neutrophils
- Biology Area
- Angiogenesis, Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, GPCR
- Antigen References
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1. Chuntharapai A, et al. 1994. J. Immunol. 153:5682.
2. Wilson S, et al. 2005. J. Biol. Chem. 280:28663.
3. Emadi S, et al. 2005. Blood 105:464.
4. Omari KM, et al. 2005. Brain 128:1003.
5. Juremalm M and Nilsson G. 2005. Chem. Immunol. Allergy 87:130.
6. Wolf M, et al. 1998. Eur. J. Immunol. 28:164. - Gene ID
- 3579 View all products for this Gene ID
- UniProt
- View information about CD182 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
- Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
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Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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