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PE anti-human CD370 (CLEC9A/DNGR1) Antibody

Pricing & Availability
Clone
8F9 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
C-type lectin family member 9A, CD370
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
8F9_PE_1_010612
Human peripheral blood mononuclear cells were stained with CD3 PerCP, HLA-DR Alexa Fluor® 488, CD141 APC, and CD370 (CLEC9A, clone 8F9) PE (top) or mouse IgG2a, κ PE isotype control (bottom). Data was analyzed with CD3-negative and HLA-DR-positive cell population.
  • 8F9_PE_1_010612
    Human peripheral blood mononuclear cells were stained with CD3 PerCP, HLA-DR Alexa Fluor® 488, CD141 APC, and CD370 (CLEC9A, clone 8F9) PE (top) or mouse IgG2a, κ PE isotype control (bottom). Data was analyzed with CD3-negative and HLA-DR-positive cell population.
  • 8F9_PE_2_010612
See PE spectral data
Cat # Size Price Save
353803 25 tests ¥33,660
353804 100 tests ¥79,200
Description

CD370 (CLEC9A), also known as DNGR1, is a type II transmembrane protein consisting of a single extracellular C-type lectin domain (CTLD) that is connected to the transmembrane domain by a stalk region, and an intracellular cytoplasmic tail with immunoreceptor tyrosine-based activation motif (ITAM). In humans, CLEC9A is strictly expressed on BDCA3-positive conventional dendritic cells (cDC) and CD16- monocytes. CLEC9A recognizes a ubiquitous preformed acid-labile protein associated ligand(s) that is exposed on necrotic cells. CLEC9A ligation is necessary for efficient cross-presentation of dead-cell-associated antigen by recruitment and activation of the tyrosine kinase Syk.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed
  2. Cytlak U, et al. 2020. Immunity. 53(2):353-370. PubMed
  3. Lee J, et al. 2015. J Exp Med. 212:385. PubMed
  4. Breton G, et al. 2015. J Exp Med. 212:401. PubMed
  5. Schrøder M, et al. 2016. PLoS One. 11: 0157387. PubMed
  6. Leach SM, et al. 2020. Cell Rep. 33:108337. PubMed
  7. Hearnden R, et al. 2021. STAR Protocols. 2(2):100422. PubMed
  8. Molina MS, et al. 2021. Front Immunol. 12:699128. PubMed
  9. Wimmers F, et al. 2021. Cell. 184:3915. PubMed
  10. Luo X, et al. 2023. Methods Mol Biol. 2618:133. PubMed
  11. Houlder E, et al. 2023. Nat Commun. 14:1863. PubMed
RRID
AB_10959651 (BioLegend Cat. No. 353803)
AB_10965546 (BioLegend Cat. No. 353804)

Antigen Details

Structure
Type II transmembrane glycoprotein; consists of a 35 amino acid (aa) cytoplasmic domain with an ITAM like motif, a 21 aa transmembrane segment, a 185 aa extracellular domain (ECD) that contains a stalk region, and one C-type lectin domain (CTLD).
Distribution

Dendritic subsets including plasmacytoid dendritic cells.

Function
Cross-present antigen to CTL, phagocytosis necrotic cells.
Interaction
Necrotic cells.
Ligand/Receptor
Ubiquitous preformed acid-labile protein associated ligand(s).
Cell Type
Dendritic cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, MHC Antigens
Antigen References

1. Poulin LF, et al. 2010. J. Exp. Med. 207:1261.
2. Sancho D, et al. 2009. Nature 458:899.
3. Huysamen C, et al. 2008. J. Biol. Chem. 283:16693.

Gene ID
283420 View all products for this Gene ID
UniProt
View information about CD370 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 01/15/2015

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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