- Clone
- 8F9 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- HCDM listed
- Other Names
- C-type lectin family member 9A, CD370
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![8F9_PE_1_010612 8F9_PE_1_010612](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/8F9_PE_1_010612.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood mononuclear cells were stained with CD3 PerCP, HLA-DR Alexa Fluor® 488, CD141 APC, and CD370 (CLEC9A, clone 8F9) PE (top) or mouse IgG2a, κ PE isotype control (bottom). Data was analyzed with CD3-negative and HLA-DR-positive cell population. -
Cat # | Size | Price | Save |
---|---|---|---|
353803 | 25 tests | ¥33,660 | |
353804 | 100 tests | ¥79,200 |
CD370 (CLEC9A), also known as DNGR1, is a type II transmembrane protein consisting of a single extracellular C-type lectin domain (CTLD) that is connected to the transmembrane domain by a stalk region, and an intracellular cytoplasmic tail with immunoreceptor tyrosine-based activation motif (ITAM). In humans, CLEC9A is strictly expressed on BDCA3-positive conventional dendritic cells (cDC) and CD16- monocytes. CLEC9A recognizes a ubiquitous preformed acid-labile protein associated ligand(s) that is exposed on necrotic cells. CLEC9A ligation is necessary for efficient cross-presentation of dead-cell-associated antigen by recruitment and activation of the tyrosine kinase Syk.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Product Citations
- RRID
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AB_10959651 (BioLegend Cat. No. 353803)
AB_10965546 (BioLegend Cat. No. 353804)
Antigen Details
- Structure
- Type II transmembrane glycoprotein; consists of a 35 amino acid (aa) cytoplasmic domain with an ITAM like motif, a 21 aa transmembrane segment, a 185 aa extracellular domain (ECD) that contains a stalk region, and one C-type lectin domain (CTLD).
- Distribution
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Dendritic subsets including plasmacytoid dendritic cells.
- Function
- Cross-present antigen to CTL, phagocytosis necrotic cells.
- Interaction
- Necrotic cells.
- Ligand/Receptor
- Ubiquitous preformed acid-labile protein associated ligand(s).
- Cell Type
- Dendritic cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, MHC Antigens
- Antigen References
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1. Poulin LF, et al. 2010. J. Exp. Med. 207:1261.
2. Sancho D, et al. 2009. Nature 458:899.
3. Huysamen C, et al. 2008. J. Biol. Chem. 283:16693. - Gene ID
- 283420 View all products for this Gene ID
- UniProt
- View information about CD370 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
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