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PE anti-human CD45 Antibody

Pricing & Availability
Clone
2D1 (See other available formats)
Regulatory Status
RUO
Other Names
Leukocyte Common Antigen (LCA), T200
Isotype
Mouse IgG1, κ
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Product Citations
publications
2D1_PE_CD45_Antibody_FC_102215
Human peripheral blood lymphocytes were stained with CD45 (clone 2D1) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • 2D1_PE_CD45_Antibody_FC_102215
    Human peripheral blood lymphocytes were stained with CD45 (clone 2D1) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
See PE spectral data
Cat # Size Price Save
368509 25 tests ¥13,420
368510 100 tests ¥30,360
Description

CD45 is a 180 - 240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes or platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human PBMC
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.

Application References

(PubMed link indicates BioLegend citation)
  1. Bradstock KF, et al. 1980. J. Natl. Cancer Inst. 65:33.
  2. Csiba A, et al. 1984. Br. J. Cancer 50:699.
  3. Tchilian EZ, et al. 2001. J. Immunol. 166:1308.
  4. Lee MS, et al. 2004. Int. Immunol. 16:1109.
Product Citations
  1. Sluzalska K,et al. 2017. Sci Rep.. 10.1038/s41598-017-14004-9. PubMed
  2. Zhang H et al. 2018. Cell. 175(5):1244-1258 . PubMed
  3. Frank AC, et al. 2019. Nat Commun. 10:1135. PubMed
  4. Zhang Y, et al. 2018. Stem Cells Int. 2018:7159465. PubMed
  5. Ali S, et al. 2018. Purinergic Signal. 14:371. PubMed
  6. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  7. Morton JJ, et al. 2018. Mol Carcinog. 57:1651. PubMed
  8. Pan C, et al. 2019. Int J Mol Med. 44:1629. PubMed
  9. Wei X, et al. 2019. Int J Mol Med. 44:1425. PubMed
  10. Sluzalska KD, et al. 2017. Osteoarthritis Cartilage. 25:1890. PubMed
  11. Cillo AR, et al. 2020. Immunity. 52(1):183-199.e9.. PubMed
  12. Yu C, et al. 2020. Sci Rep. 10:14521. PubMed
  13. Yu LC, et al. 2020. Front Neurol. 1.104166667. PubMed
  14. Rai MA, et al. 2020. Retrovirology. 17:35. PubMed
  15. Zhuang J, et al. 2020. Nucleic Acids Res. 48:8870. PubMed
  16. Chioh FW, et al. 2021. eLife. 10:00. PubMed
  17. Tidu F, et al. 2021. iScience. 24(6):102683. PubMed
  18. Dam T, et al. 2021. Front Physiol. 11:613367. PubMed
  19. Delvecchio FR, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:1543. PubMed
  20. Chan JM, et al. 2021. Cancer Cell. 39:1479. PubMed
  21. Meng YM, et al. 2021. Nat Commun. 12:6011. PubMed
  22. Yao X, et al. 2022. Bioeng Transl Med. 7:e10250. PubMed
  23. Sonoda S, et al. 2022. STAR Protoc. 3:101386. PubMed
  24. Liu Y, et al. 2022. iScience. 25:104405. PubMed
  25. Li Y, et al. 2021. Aging (Albany NY). 13:25886. PubMed
  26. Boya M, et al. 2022. Nat Commun. 13:3385. PubMed
  27. Zhang X, et al. 2022. Nat Commun. 13:5211. PubMed
  28. Dong N, et al. 2022. World J Stem Cells. 14:556. PubMed
  29. Quintanal-Villalonga A, et al. 2022. STAR Protoc. 3:101776. PubMed
  30. Sasamoto Y, et al. 2022. Cell Rep. 40:111166. PubMed
  31. Ding L, et al. 2023. J Clin Invest. 133: . PubMed
  32. Cillo AR, et al. 2022. Clin Cancer Res. 28:4968. PubMed
  33. Shiroshita K, et al. 2022. Cell Rep Methods. 2:100354. PubMed
  34. Chang L, et al. 2023. Life Sci Alliance. 6:. PubMed
  35. Nisa BU, et al. 2023. Life (Basel). 13:. PubMed
  36. Seree-Aphinan C, et al. 2023. Front Immunol. 14:1138765. PubMed
  37. Molpeceres C, et al. 2023. Int J Bioprint. 9:720. PubMed
  38. Wu L, et al. 2023. Cell Res. . PubMed
RRID
AB_2566369 (BioLegend Cat. No. 368509)
AB_2566370 (BioLegend Cat. No. 368510)

Antigen Details

Structure
Type I transmembrane protein, 180 - 240 kD (multiple isoforms)
Distribution

Hematopoietic cells, except circulating erythrocytes or platelets.

Function
Tyrosine phosphatases, signaling, co-stimulation (co-inhibition), TCR and BCR mediated activation.
Ligand/Receptor
Galectin-1, CD2, CD3, and CD4.
Cell Type
B cells, Dendritic cells, Neutrophils
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Protein Kinases/Phosphatase, TCRs
Antigen References

1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol. 12:85.

Gene ID
5788 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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