- Clone
- S16009A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Prf1, Pfp, Pfn, Pfn-1
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![S16009A_PE_Perforin_Antibody_1_112217 S16009A_PE_Perforin_Antibody_1_112217](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/S16009A_PE_Perforin_Antibody_1_112217.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 splenocytes were stained with CD49b APC. Cells were fixed, permeabilized, and stained with PE anti-mouse perforin (clone S16009A, top) or PE rat IgG2a, κ isotype control (bottom). -
Cat # | Size | Price | Save |
---|---|---|---|
154305 | 25 µg | ¥31,020 | |
154306 | 100 µg | ¥81,840 |
Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.
Product DetailsProduct Details
- Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse perforin transfected cells.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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ICFC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
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Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
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Clone S16009A reacts with perforin from both C57BL/6 and BALB/c strains.
- Product Citations
- RRID
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AB_2721638 (BioLegend Cat. No. 154305)
AB_2721639 (BioLegend Cat. No. 154306)
Antigen Details
- Structure
- 70 kD Protein that oligomerizes for pore formation.
- Distribution
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CTL, NK (Cytoplasmic Granules)
- Function
- Target cell lysis
- Cell Type
- NK cells, T cells
- Biology Area
- Immunology
- Molecular Family
- Cytokines/Chemokines
- Antigen References
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- Podack ER, et al. 1988. Immunol. Rev. 103:203.
- Konjar S, et al. 2010. Immunology. 131:257.
- Trapani JA, et al. 2002. Nat. Rev. Immunol. 2:735.
- Osinska I, et al. 2014. Cent. Eur. J. Immnol. 39:109.
- Gene ID
- 18646 View all products for this Gene ID
- UniProt
- View information about Perforin on UniProt.org
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
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