- Clone
- A15158B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Signal transducer and activator of transcription 1 (STAT1), Transcription factor ISGF-3 components p91/p84
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A15158B_PE_STAT1_Antibody_1_062216 A15158B_PE_STAT1_Antibody_1_062216](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A15158B_PE_STAT1_Antibody_1_062216.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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HeLa cells were stimulated with (filled histogram) or without (open histogram) nocodozole for 24 hours, fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, then intracellularly stained with anti-STAT1 Phospho (Ser727) antibody (clone A15158B) PE. -
Human peripheral blood lymphocytes were stimulated with (filled histogram) or without (open histogram) Cell Activation Cocktail (without Brefeldin A) for 15 minutes, fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, then intracellularly stained with anti-STAT1 Phospho (Ser727) antibody (clone A15158B) PE. -
NIH/3T3 cells were treated with (filled histogram) or without (open histogram) nocodozole for 24 hours, fixed with Fixation Buffer (Cat No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat No. 425401), then intracellularly stained with anti-STAT1 Phospho (Ser727) antibody (clone A15158B) PE.
Cat # | Size | Price | Save |
---|---|---|---|
686403 | 25 tests | ¥40,260 | |
686404 | 100 tests | ¥92,400 |
STAT1, also known as signal transduction and activator of transcription 1, is a ubiquitously expressed cytoplasmic protein and is activated in response to cytokine signaling, including IFN-α, IFN-γ, EGF, PDGF, and IL-6. Upon activation, STAT1 is phosphorylated by receptor-associated kinases, translocates to the nucleus, and functions as a transcription factor. Two isoforms of STAT1, with apparent molecular weights of 88 and 91 kD, exist as a result of alternative RNA processing. STAT1 is involved in IFN-mediated immune responses, and STAT1-deficient mice are highly sensitive to bacterial and viral infections.
Product DetailsProduct Details
- Reactivity
- Human,Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human STAT1 peptide phosphorylated at Ser 727.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
For staining mouse cells a higher volume than 5 µl may be required. - Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Product Citations
- RRID
-
AB_2616938 (BioLegend Cat. No. 686403)
AB_2616939 (BioLegend Cat. No. 686404)
Antigen Details
- Structure
- 750 amino acids, predicted molecular weight of 87 kD; contains a SH2 domain responsible for homodimerization or heterodimerization.
- Distribution
-
Translocates to the nucleus when phosphorylated.
- Function
- Phosphorylated in response to cytokine signaling by receptor-associated kinases; translocates to the nucleus to act as a transcription factor. Mediates responses to type I (IFN-α/β) and II interferon (IFN-γ), EGF, PDGF, and IL-6.
- Interaction
- Forms a homodimer or heterodimers with other family members. Interacts with FAK, MCM3, MCM5, TRADD, BRCA1, KIT, IL-27R, IL-2Rβ, IL-2Rγ, IFNαβR, and c-Src.
- Biology Area
- Cell Biology, Signal Transduction
- Molecular Family
- Nuclear Markers, Phospho-Proteins
- Antigen References
-
1. Durbin JE, et al. 1996. Cell. 84:443.
2. Darnell JE Jr, et al. 1994. Science 264:1415.
3. Chen X, et al. 1998. Cell. 93:827.
4. Ramana CV, et al. 2000. Oncogene. 19:2619. - Gene ID
- 6772 View all products for this Gene ID
- UniProt
- View information about STAT1 Phospho Ser727 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
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