- Clone
- Ber-ACT35 (ACT35) (See other available formats)
- Regulatory Status
- RUO
- Other Names
- ACT35 antigen, tumor necrosis factor receptor superfamily member 4 (TNFRSF4)
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![Ber-ACT35_PECy7_1_072811 Ber-ACT35_PECy7_1_072811](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/Ber-ACT35_PECy7_1_072811.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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PHA-stimulated (3 days) human peripheral blood lymphocytes were stained with CD4 FITC and OX-40 (clone Ber-ACT35) PE/Cyanine7 (top) or mouse IgG1, κ PE/Cyanine7 isotype control (bottom). -
Cat # | Size | Price | Save |
---|---|---|---|
350012 | 100 tests | ¥68,420 |
CD134, also known as OX40 and TNFRSF4, is a 50 kD type I transmembrane glycoprotein. It is a member of the TNF receptor family. OX40 is expressed on activated T lymphocytes including Th1, Th2, Th17, and Treg cells. The interaction of OX40 with OX40L results in B cell proliferation and antibody secretion, regulation of primary T cell expansion, and T cell survival. OX40 influences the size of the T cell memory pool and regulation of CD4+ T cell tolerance.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- HTLV 1-transformed HUT 102 cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
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Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
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Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, immunohistochemical staining2,3of paraffin embedded7 and frozen tissue sections, ELISA4, and functional assay5. The LEAF™ or Ultra-LEAF™ purified antibody is recommended for functional assays (contact our custom solutions team).
- Additional Product Notes
- BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
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Application References
(PubMed link indicates BioLegend citation) -
- Latza U, et al. 1994. Eur. J. Immunol. 24:677. (WB, IP)
- Durkop H, et al. 1995. Brit. J. Haematol. 91:927. (IHC)
- Durkop H, et al. 1997. Brit. J. Haematol. 98:863. (IHC)
- Willett B, et al. 2007. J. Virol. 81:9665. (ELISA)
- Li M and Zhang Y. et al. 2005. Cell. Mol. Immunol. 2:467. (FA)
- Gloviczki ML, et al. 2012. Clin. J. Am. Soc. Nephrol. 8:546. PubMed
- Domingos PL, et al. 2012. An. Bras. Dermatol. 87:851. (IHC)
- Product Citations
- RRID
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AB_10901161 (BioLegend Cat. No. 350012)
Antigen Details
- Structure
- 50 kD, TNF receptor family
- Distribution
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Activated T cells
- Function
- Receptor for OX40 ligand, co-stimulatory signal for T cell proliferation and survival.
- Ligand/Receptor
- OX40L (CD252)
- Cell Type
- T cells, Tregs
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
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1. Smith CA, et al. 1994. Cell. 76:959.
2. Chen AL, et al. 1999. Immunity. 11:689.
3. Croft M. 2010. Annu. Rev. Immunol. 28:57.
4. Withers DR, et al. 2009. J. Immunol. 183:5079.
5. Klinger M, et al. 2009. J. Immunol. 182:4581. - Gene ID
- 7293 View all products for this Gene ID
- UniProt
- View information about CD134 on UniProt.org
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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