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PE/Cyanine7 anti-human CD3 Antibody

Pricing & Availability
Clone
UCHT1 (See other available formats)
Regulatory Status
RUO
Workshop
III 471
Other Names
T3, CD3ε
Isotype
Mouse IgG1, κ
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Product Citations
publications
UCHT1_PECy7_111407
Human peripheral blood lymphocytes stained with UCHT1 PE/Cyanine7 and overlayed with isotype control
  • UCHT1_PECy7_111407
    Human peripheral blood lymphocytes stained with UCHT1 PE/Cyanine7 and overlayed with isotype control
See PE/Cyanine7 spectral data
Cat # Size Price Save
300419 25 tests ¥25,460
300420 100 tests ¥49,490
Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NKT cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
  2. Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
  3. Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
  4. Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
  5. Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
  6. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
  7. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  8. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  9. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
  10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  11. Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)
  12. Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed
  13. Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
  14. Li J, et al. 2015. Cancer Res. 75:508. PubMed
  15. Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG)
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Taraldsrud E, et al. 2017. Journal of Autoimmunity. 10.1016/j.jaut.2017.04.004. PubMed
  2. Verma K, et al. 2017. PLoS One.. 10.1371/journal.pone.0183828. PubMed
  3. Gladkikh A, et al. 2017. Cancer Med. . 10.1002/cam4.1257. PubMed
  4. Howson LJ, et al. 2018. Nat Commun. 9:253. PubMed
  5. Palazzo A, et al. 2018. J Immunol. 200:2304. PubMed
  6. Ickrath P, et al. 2019. Biomed Rep. 10:119. PubMed
  7. Meraviglia S, et al. 2017. Oncoimmunology. 6:e1347742. PubMed
  8. Leone DA, et al. 2017. J Immunol. 199:531. PubMed
  9. Iio K, et al. 2019. Sci Rep. 9:813. PubMed
  10. Suan D et al. 2017. Immunity. 47(6):1142-1153 . PubMed
  11. Goel G, et al. 2019. Sci Adv. 5:eaaw7756. PubMed
  12. Ickrath P, et al. 2018. Int J Mol Med. 42:1116. PubMed
  13. Manser AR, et al. 2019. J Immunol. 203:2301. PubMed
  14. Mayassi T, et al. 2019. Cell. 176:967. PubMed
  15. Lamichhane R et al. 2019. Cell Rep. 28(12):3061-3076 . PubMed
  16. Román-Rodriguez FJ, et al. 2019. Cell Stem Cell. 25:607. PubMed
  17. Pollack RA, et al. 2017. Cell Host Microbe. 1.218055556. PubMed
  18. Beyer AI, et al. 2017. Stem Cells Dev. 26:102. PubMed
  19. Rosskopf S, et al. 2018. Oncotarget. 9:17608. PubMed
  20. Di Blasi D, et al. 2019. Cell Mol Gastroenterol Hepatol. 0.510416667. PubMed
  21. Weisberg SP, et al. 2020. Cell Reports. 29(12):3916-3932.e5.. PubMed
  22. Zhao Y, et al. 2020. Front Immunol. 2.572222222. PubMed
  23. Moore N, et al. 2019. Sci Rep. 9:15101. PubMed
  24. Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
  25. Vela M, et al. 2019. Sci Rep. 9:18792. PubMed
  26. Cartellieri M, et al. 2014. PLoS One. 9:93745. PubMed
  27. Mashiko S, et al. 2015. J Allergy Clin Immunol. 136: 351-359. PubMed
  28. Ishigaki K, et al. 2015. Sci Rep. 5: 12937. PubMed
  29. Petit N, et al. 2015. PLoS One. 10: 0138420. PubMed
  30. Leone D, et al. 2016. PLoS One. 11: 0151674. PubMed
  31. Nagafuchi Y, et al. 2016. Sci Rep. 6:29338. PubMed
  32. Seumois G, et al. 2016. J Immunol. 197: 655 - 664. PubMed
  33. Usero L, et al. 2016. Diabetes. 65: 2356 - 2366. PubMed
  34. Comte D, et al. 2016. Proc Natl Acad Sci U S A. 113: 9321 - 9326. PubMed
  35. Cartellieri M, et al. 2016. Blood Cancer J. 6:e458. PubMed
  36. Zeng J, et al. 2020. Nat Med. 26:535. PubMed
  37. Todorova D, et al. 2020. EBioMedicine. 62:103120. PubMed
  38. Andreano E, et al. 2021. Cell. 184(7):1821-1835.e16. PubMed
  39. Shevyrev D, et al. 2021. Exp Ther Med. 209:21. PubMed
  40. Sandborn WJ, et al. 2021. Gastroenterology. 161:1853. PubMed
  41. Chruewkamlow N, et al. 2021. Stem Cell Res Ther. 12:520. PubMed
  42. Andreano E, et al. 2021. Nature. Online ahead of print. PubMed
  43. Jiang L, et al. 2021. Int J Biol Sci. 17:4377. PubMed
  44. Vijayakumar B, et al. 2022. Immunity. . PubMed
  45. Ramirez NP, et al. 2022. Nat Commun. 13:1109. PubMed
  46. Gustafson CE, et al. 2020. JCI Insight. 5:. PubMed
  47. Brunk F, et al. 2021. Eur J Immunol. 51:2651. PubMed
  48. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  49. Lamichhane R, et al. 2020. Eur J Immunol. 50:178. PubMed
  50. Ridley ML, et al. 2020. J Immunol. 204:2940. PubMed
  51. Vinjamur DS, et al. 2021. Nat Genet. 53:719. PubMed
  52. Bonte S, et al. 2021. Oncoimmunology. 10:1954800. PubMed
  53. Teng F, et al. 2021. Cell Rep. 37:110051. PubMed
  54. Warmuth S, et al. 2022. Oncoimmunology. 10:2004661. PubMed
  55. Alonso R, et al. 2022. Blood Adv. :. PubMed
  56. Lo Presti E, et al. 2020. J Leukoc Biol. 108:749. PubMed
  57. Cruz-Lorenzo E, et al. 2022. Viruses. 14:. PubMed
  58. Hu Z, et al. 2022. Nat Metab. 4:759. PubMed
  59. Vazquez-Lombardi R, et al. 2022. Immunity. 55:1953. PubMed
  60. Balta E, et al. 2023. Front Immunol. 13:1063313. PubMed
  61. Andreano E, et al. 2023. Nat Commun. 14:53. PubMed
  62. Miller RA, et al. 2022. J Immunother Cancer. 10: . PubMed
  63. Iio K, et al. 2023. Immun Ageing. 20:8. PubMed
  64. Dong W, et al. 2023. Int J Mol Med. 51: . PubMed
  65. Blommaert E, et al. 2022. Hum Genet. 141:1279. PubMed
  66. Zhang C, et al. 2023. Front Immunol. 14:1182409. PubMed
  67. Shaw DG, et al. 2023. Nat Med. 29:1520. PubMed
RRID
AB_439780 (BioLegend Cat. No. 300419)
AB_439781 (BioLegend Cat. No. 300420)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

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Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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