- Clone
- 5C3 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- V CD40.4
- Other Names
- BP50, TNFRSF5
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![5C3_PECyanine7_CD40_Antibody_1_073124 5C3_PECyanine7_CD40_Antibody_1_073124](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/5C3_PECyanine7_CD40_Antibody_1_073124.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were stained with anti-human CD19 (clone HIB19) FITC and anti-human CD40 (clone 5C3) PE/Cyanine7 (left) or mouse IgG1, κ PE/Cyanine7 isotype control (right).
Cat # | Size | Price | Save |
---|---|---|---|
334321 | 25 tests | ¥31,020 | |
334322 | 100 tests | ¥67,100 |
CD40 is a 48 kD type I glycoprotein also known as BP50. It is a member of the TNFR superfamily primarily expressed on B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts, and at low levels on plasma cells. CD40 has been reported to be involved in B cell differentiation, costimulation, isotype class-switching, and protection of B cells from apoptosis. Additionally, CD40 is important for T cell-B cell interactions. The ligand of CD40 is CD154 (CD40 ligand). The 5C3 antibody has been reported to promote B cell proliferation in the presence of anti-IgM, IL-4 or PMA, partially blocking CD40 binding to CD40L, and B cells rescue from apoptosis.
Product DetailsProduct Details
- Reactivity
- Human,Cynomolgus,Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
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Additional reported applications (for the relevant formats) include: costimulation of B cell proliferation1, partial inhibition of CD40 binding to CD40L3, and B cell rescue from apoptosis1. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays.
- Additional Product Notes
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BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
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Application References
(PubMed link indicates BioLegend citation) -
- Schlossman SF, et al. 1995. ed. Leukocyte Typing V:White Cell Differentiation Antigens. New York:Oxford University Press.
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Pound JD, et al. 1999. Int. Immunol. 11:11.
- Shey MS, et al. 2014. J Immunol. 192:4833. PubMed
- Sondergaard JN, et al. 2014. Mol Immunol. 59:180. PubMed
- Saiki O, et al. 1995. J Clin. Invest. 2: 510-4 (Costim)
- Product Citations
- RRID
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AB_10643414 (BioLegend Cat. No. 334321)
AB_10645472 (BioLegend Cat. No. 334322)
Antigen Details
- Structure
- TNFR superfamily, type I glycoprotein, 48 kD
- Distribution
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B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts
- Function
- B cell differentiation, costimulation, isotype class-switching, rescues B cells from apoptosis
- Ligand/Receptor
- CD154 (CD40 ligand)
- Cell Type
- B cells, Dendritic cells, Endothelial cells, Fibroblasts, Macrophages
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
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1. Banchereau J, et al. 1994. Annu. Rev. Immunol. 12:881.
2. Foy T, et al. 1996. Annu. Rev. Immunol. 14:591. - Gene ID
- 958 View all products for this Gene ID
- UniProt
- View information about CD40 on UniProt.org
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